Screening for estrogen active nonylphenols in surface waters by planar solid phase extraction-planar yeast estrogen screen.

This study presents a new sensitive and innovative screening approach for estrogen active NP based on high-performance thin-layer chromatography. NP were focused in a single target zone on thin-layer plates by planar solid phase extraction (pSPE) and detected by a planar yeast estrogen screen (pYES) on the basis of their estrogenic activity. The mean limits of detection and quantitation were 14 and 26 ng per zone, respectively. After liquid-liquid extraction of water samples with dichloromethane, the mean recovery was close to 100% (relative standard deviation of 21% or less), and estrogen active NP were detectable down to 1 μg/L. Thus, pSPE-pYES provides both the detection and the quantitation of estrogenic NP in surface waters at the maximum acceptable concentration. Application of the approach on extracts of surface waters showed the use of pSPE-pYES for environmental samples, and no complex and time-consuming clean-up of the extracts was required. Estrogenic NP were not detectable in any of the investigated surface waters by means of the screening approach presented. PMID: 31420710 [PubMed - as supplied by publisher]
Source: Analytical and Bioanalytical Chemistry - Category: Chemistry Authors: Tags: Anal Bioanal Chem Source Type: research