Ovarian Cholesterol Efflux: ABC Transporters and Follicular Fluid HDL Regulate Cholesterol Content in Mouse Oocytes.

Ovarian Cholesterol Efflux: ABC Transporters and Follicular Fluid HDL Regulate Cholesterol Content in Mouse Oocytes. Biol Reprod. 2019 Aug 19;: Authors: Quiroz A, Molina P, Santander N, Gallardo D, Rigotti A, Busso D Abstract High density lipoproteins (HDL) take up cholesterol from peripheral tissues via ABC transporters and deliver it to the liver via scavenger receptor class B type I (SR-B1), for its biliary excretion. Evidence from different mammals suggests that HDL metabolism may influence female reproduction. Indeed, HDL are the main lipoproteins in follicular fluid (FF) and their composition associates to preimplantation embryo quality. The origin of FF HDL is controversial: FF HDL are thought to derive from plasma via diffusion through the basal lamina of the follicle but their molecular structure is different from plasma HDL. SR-B1 knock-out (KO) mice have provided important evidence linking HDL metabolism and female fertility. These mice have large, cholesterol-rich circulating HDL. Females are infertile, and their fertility is restored by probucol treatment, which lowers plasma cholesterol. We recently showed that ovulated oocytes from SR-B1 KO mice have excess cholesterol and are dysfunctional. However, the mechanisms explaining how high plasma HDL cholesterol relates to cholesterol excess in oocytes, and if FF HDL regulates oocyte cholesterol homeostasis are unknown. In this work, we showed that cholesterol excess in ooc...
Source: Biology of Reproduction - Category: Reproduction Medicine Authors: Tags: Biol Reprod Source Type: research