Influence of dietary supplementation with Bacillus velezensis on intestinal microbial diversity of mice

Publication date: Available online 19 August 2019Source: Microbial PathogenesisAuthor(s): Aoyun Li, Yaping Wang, Lulu Pei, Khalid Mehmood, Kun Li, Hammad Qamar, Mudassar Iqbal, Muhammad Waqas, Juanjuan Liu, Jiakui LiAbstractYaks are an aboriginal breed of the Qinghai-Tibet plateau (3000 m), which are highly adaptable to cold and hypoxic environments. It is noticed that hypoxia and hypothermia can induce changes in intestinal microbial structure in animals. Increasing evidences suggested that probiotics supplementation can improve the balance of gut microbiota of animals. However, so far, very few studies have emphasized on the probiotics isolated from yaks in the Qinghai-Tibet Plateau. Therefore, a potential probiotic strain Bacillus velezensis was isolated from yaks. In the present study, a total of 18 Kunming mice (15–18 g) were equally distributed into two groups; control and probiotic treated groups (1 × 109 CFU/day). During the experimental period, All the mice from both groups were given standard normal diet ad libitum. At the end of the experiment, mice were sacrificed and the intestines (duodenum, jejunum, ileum, and cecum) were removed for high-throughput sequencing. The results demonstrated that Bacillus velezensis supplementation showed beneficial effects on the gut microbiota of mice. Specifically, Bacillus velezensis supplementation increased the population of Lactobacillus and Ruminococcus in the duodenum, and Candidatus Arthromitus in the jejunum. ...
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research