Fluorescent activity-based probe for the selective detection of Factor VII activating protease (FSAP) in human plasma

The zymogen form of circulating Factor VII activating protease (FSAP) is activated by histones that are released as a consequence of tissue damage or excessive inflammation. This is likely to have consequences in a number of disease conditions such as stroke, atherosclerosis, liver fibrosis, thrombosis and cancer. To investigate the existence, as well as the concentration, of active FSAP (FSAPa) in complex biological systems an active site probe is needed. We used Hybrid Combinatorial Substrate Library (HyCoSuL) to screen for natural and unnatural amino acids that specifically bind to P4-P2 pockets of FSAPa.

Source: Thrombosis Research - August 18, 2019 Category: Hematology Authors: Wioletta Rut, Nis Valentin Nielsen, Justyna Czarna, Marcin Poreba, Sandip M. Kanse, Marcin Drag Tags: Full Length Article Source Type: research