Physiological and proteomic response of Escherichia coli O157:H7 to a bioprotective lactic acid bacterium in a meat environment

Publication date: Available online 17 August 2019Source: Food Research InternationalAuthor(s): Alejandra Orihuel, Lucrecia Terán, Jenny Renaut, Sébastien Planchon, María Pía Valacco, Emilse Masias, Carlos Minahk, Graciela Vignolo, Silvia Moreno, André M. De Almeida, Lucila Saavedra, Silvina FaddaAbstractThe enterohemorrhagic Escherichia (E.) coli (EHEC) is a pathogen of great concern for public health and the meat industry all over the world. The high economic losses in meat industry and the high costs of the illness highlight the necessity of additional efforts to control this pathogen. Previous studies have demonstrated the inhibitory activity of Enterococcus mundtii CRL35 towards EHEC, showing a specific proteomic response during the co-culture. In the present work, additional studies of the EHEC-Ent. mundtii interaction were carried out: i) differential protein expression of E. coli O157:H7 NCTC12900 growing in co-culture with Ent. mundtii in a meat environment, ii) the reciprocal influence between these two microorganisms in the adhesion to extracellular matrix (ECM) proteins and iii) the possible induction of the phage W933, coding for Shiga toxin (Stx1), by Ent. mundtii CRL35. Proteomic analysis showed a significant repression of a number of E. coli NCTC12900 proteins in co-culture respect to its single culture, these mostly related to the metabolism and transport of amino acids and nucleotides. On the other hand, statistically signif...
Source: Food Research International - Category: Food Science Source Type: research

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Publication date: Available online 17 September 2019Source: American Journal of Infection ControlAuthor(s): Jyothi Manohar, Savannah Hatt, Brigette B. DeMarzo, Freida Blostein, Anna E.W. Cronenwett, Jianfeng Wu, Kyu Han Lee, Betsy FoxmanBackgroundUrinary catheterization, even of short duration, increases the risk of subsequent urinary tract infection (UTI). Whether the bacteria found on the surface of catheters placed for
Source: American Journal of Infection Control - Category: Infectious Diseases Source Type: research
In this study, the effects of COS on colorectal cancer (CRC) development were evaluated using azoxymethane and dextran sulfate sodium (AOM/DSS) induced mouse model of CRC (CACM). In the COS-treated CRC group (CMCOS), COS protected mice from CRC by decreasing the disease activity index, tumor incidences and multiplicity, and the mRNA levels of COX-2, IL-6, TNF-α, IL-1β, IL-10, and IKK-β mRNA in colonic epithelial cells. The results of a cage-exchanged experiment, in which mice from the CACMe and CMCOSe treatments exchanged cages every day to interact with microbes, showed that gut microbes play an important ...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Updated Date: Fri, 13 Sep 2019 00:00:00 EDT
Source: DailyMed Drug Label Updates - Category: Drugs & Pharmacology Source Type: alerts
ConclusionsThe intestinal microbiota of AIDS/HIV patients were disordered, and there was a correlation between the amount of intestinal flora and the number of CD4+ T lymphocytes and the levels of TNF- α and IL-6.
Source: Journal of Zhejiang University. Science. B. - Category: Universities & Medical Training Source Type: research
CONCLUSIONS: The intestinal microbiota of AIDS/HIV patients were disordered, and there was a correlation between the amount of intestinal flora and the number of CD4+ T lymphocytes and the levels of TNF-α and IL-6. PMID: 31489799 [PubMed - in process]
Source: J Zhejiang Univ Sci ... - Category: Science Authors: Tags: J Zhejiang Univ Sci B Source Type: research
ConclusionsTopical vancomycin within the surgical site is not correlated with vancomycin-resistant bacteria. However, there was an increased incidence of gram-negative organisms in infections after vancomycin application in the wound versus no vancomycin. Continued surveillance with prospectively collected randomized data is necessary to better understand bacterial evolution against current antimicrobial techniques.Level of EvidenceLevel III.
Source: Spine Deformity - Category: Orthopaedics Source Type: research
Publication date: Available online 5 September 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): Sana Fatima, Akhtar Rasool, Nasreena Sajjad, Eijaz Ahmed Bhat, Marlia Mohd Hanafiah, Mohammed MahboobAbstractPenicillin is an antibiotic drug effective against many serious diseases such as syphilis and Staphylococcus infections. Different kinds of fungi have used to obtain various biotechnological products. The current study elucidates the qualitative and quantitative analysis of Penicillin derived from soil fungi using rapid High-performance liquid chromatography (HPLC) when procreating on different growth con...
Source: Biocatalysis and Agricultural Biotechnology - Category: Biotechnology Source Type: research
This study provides reference data for the design of clinically effective antibacterial peptides.
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research
CONCLUSION: The observed antibacterial experimental data indicates the selectivity of our synthesized conjugates against E.Coli. The protecting group on amino acids decreases the antibacterial activity. The synthesized conjugates are non-toxic to the normal cell lines. The experimental data were supported by computational studies. PMID: 31483233 [PubMed - as supplied by publisher]
Source: Medicinal Chemistry - Category: Chemistry Authors: Tags: Med Chem Source Type: research
Conclusion: The rates of positive bile samples were significantly higher in 2016. The profile of the most frequent pathogens was the same. The efficacy of the first-choice empirical AB ciprofloxacine decreased in 2016. The types of the most frequent cholangitis-causing bacteria are in line with the ones included in the Tokyo Guideline. Orv Hetil. 2019; 160(36): 1437-1442. PMID: 31492084 [PubMed - in process]
Source: Orvosi Hetilap - Category: General Medicine Authors: Tags: Orv Hetil Source Type: research
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