Effect of single and repeated administration of amitriptyline on neuropathic pain model in rats: Focus on glutamatergic and upstream nitrergic systems

Publication date: Available online 12 August 2019Source: Life SciencesAuthor(s): Hanan S.M. Farghaly, Mohamed M. Elbadr, Marwa A. Ahmed, Azza S. AbdelhaffezAbstractAimsFew studies have compared the interaction of single and repeated administration of amitriptyline (amit) with the nitrergic system and glutamatergic system in the experimental model of neuropathic pain. We aimed to evaluate the antinociceptive effect of single and repeated administration of amit and to assess whether glutamate preceded inducible nitric oxide synthase (iNOS) inhibition as a mechanism of the analgesic effect of amit in the neuropathic model of pain.Materials and methodsMale Wistar rats were subjected to left sciatic nerve ligation. The effect of single (25 mg kg−1) and repeated (10 mg kg−1 daily for 3 weeks) administration of amit intraperitoneally (i.p.) alone or in combination with aminoguanidine (AG i.p., 100 mg kg−1 for 3 days, a selective iNOS inhibitor) and MK-801 (0.05 mg kg−1 i.p., NMDA antagonist) on resting paw posture and mechanical hyperalgesia were studied. Glutamate level and iNOS protein expression in hippocampus were detected.Key findingsSingle and repeated administration of amit alone or in combination with AG or MK-801 demonstrated a significant decrease in resting pain score and increase in the pain threshold. Both glutamate and nitrite levels decreased in the hippocampi of single and repeated amit + MK-801 groups. Immunohistoche...
Source: Life Sciences - Category: Biology Source Type: research

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Authors: Martínez-Acitores D, Hernández Ainsa M, Cortés García L, Bengochea Martínez ML, Palacios Fanlo MJ Abstract Ectopic hepatocellular carcinoma is a rare entity. Most cases are asymptomatic and are occasionally found during autopsy or laparoscopy. They may sometimes cause relevant clinical problems such as abdominal pain or intra-abdominal bleeding. In this clinical case report, we review the literature in order to decipher the case of a 68-year-old female with an ectopic hepatocellular carcinoma that arose from the peritoneum. The patient was diagnosed after being studied du...
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