Urinary metabolic disturbance in the olfactory bulbectomized rats and the modulatory effects of fluoxetine

Publication date: Available online 12 August 2019Source: Life SciencesAuthor(s): Yun-Feng Zhou, Li Feng, Xin-Min Liu, Xue Tao, Li-Sha Wang, Meng-Di Zhang, Zhi Wang, Shan-Guang Chen, Qi ChangAbstractAimsThe present study aims to investigate the impacts of olfactory bulbectomy (OBX) on urinary metabolic profile and tryptophan metabolites in prefrontal cortex (PFC) of rats, and to explore the regulation effects of fluoxetine.Main methodsOBX model was developed by aspiration of olfactory bulbs. After fluoxetine treatment (10 mg/kg) for 14 days, urine samples were collected and behavior tests were applied. Tryptophan (TRP) metabolites and neurotransmitters in PFC were determined by prominence ultrafast liquid chromatography-QTRAP-mass spectrometry, and tryptophan hydroxylase 2 (TPH2) and indoleamine-2,3-dioxygenase 1 (IDO1) were evaluated by western blot. Urinary metabolites were analyzed by ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry-based metabonomics strategy.Key findingOBX rats showed hyperlocomotion in open field, hyperactivity in open arm and despair status, and fluoxetine reserved these behavioral abnormalities. The levels of TRP, 5-HIAA, 5-HIAA/5-HT ratio and DA increased, while kynurenine and 5-HT decreased in PFC of OBX rats. The activities of TPH2 and IDO1were inhibited after OBX. Twenty-six altered metabolites were identified as potential biomarkers in OBX rats involved in tryptophan metabolism, gut microbiota me...
Source: Life Sciences - Category: Biology Source Type: research

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Source: Neurochemical Research - Category: Neuroscience Authors: Tags: Neurochem Res Source Type: research
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Source: Frontiers in Psychiatry - Category: Psychiatry Source Type: research
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