Proteomic analysis of the intestine reveals SNARE-mediated immunoregulatory and amino acid absorption perturbations in a rat model of depression

Publication date: Available online 17 August 2019Source: Life SciencesAuthor(s): Xue Gong, Cheng Huang, Xun Yang, Qiang Mao, Li Zeng, Peng Zheng, Juncai Pu, Jianjun Chen, Haiyang Wang, Bing Xu, Chanjuan Zhou, Peng XieAbstractAimsTo clarify the role of the gut-brain axis in depression.Main methodsWe used the iTRAQ technique to identify differential proteins in the intestine of the rat model of chronic unpredictable mild stress (CUMS)-induced depression. Significant differential proteins were subjected to Gene Ontology (GO) functional annotations and KEGG pathway enrichment analysis. Key proteins were validated at the mRNA and protein levels. The levels of cytokines in the intestine, serum and hypothalamus were examined by ELISA. HPLC-UV was used to detect the levels of amino acids.Key findingsIn the rat intestine, 349 differential proteins (209 downregulated, 140 upregulated) were identified. GO analysis indicated that “protein complex assembly” was the first-ranked biological process. SNARE complex components, including SNAP23, VAMP3 and VAMP8, were increased at the mRNA levels, while only VAMP3 and VAMP8 were also upregulated at the protein level. TNFα, IL6 and IL1β were upregulated in the CUMS rat intestine, while TNFα was decreased in the serum and hypothalamus. IL1β was decreased in the serum. “Protein digestion and absorption” was the most significantly enriched KEGG pathway, involving 5 differential proteins: SLC9A3, ANPEP, ...
Source: Life Sciences - Category: Biology Source Type: research

Related Links:

Conclusion: Based on these results NDDS is considered to be an applicable instrument for identifying personality pathology in patients with depressive symptoms, by recognizing the specific pattern. This is thought to be important for adequate treatment planning. PMID: 31517547 [PubMed - as supplied by publisher]
Source: Nordic Journal of Psychiatry - Category: Psychiatry Tags: Nord J Psychiatry Source Type: research
Depression, schizophrenia and some other mental health conditions have a variety of symptoms but they all seem to be connected by similar differences in the brain
Source: New Scientist - Health - Category: Consumer Health News Source Type: research
Publication date: Available online 11 September 2019Source: Food HydrocolloidsAuthor(s): Chao-Feng Hsieh, James N. BeMiller, Kerry C. HuberAbstractGranular and molecular reaction patterns of maize and wheat starches (normal and waxy genotypes) were investigated in relation to extent of granule hydration (equilibrated at 25, 55, 75, 86 or 100% relative humidity [RH]; moisture range ≈ 10–40 g/100 g starch) within a model reaction system utilizing a fluorescent reagent (5-(4,6-dichlorotriazinyl)aminofluorescein). The greatest incremental increase in granule hydration (49–80%) and overall extent of re...
Source: Food Hydrocolloids - Category: Food Science Source Type: research
PMID: 31512733 [PubMed - in process]
Source: Tijdschrift voor Psychiatrie - Category: Psychiatry Authors: Tags: Tijdschr Psychiatr Source Type: research
Publication date: Available online 13 September 2019Source: Social Science ResearchAuthor(s): Wensong ShenAbstractResearch on depression and education usually focuses on a unidirectional relationship. This paper proposes a reciprocal relationship, simultaneously estimating the effects of depression on education and of education on depression. China, which has the world's largest education system, is used as a case study. This paper applies structural equation modeling to three datasets: the China Family Panel Studies, the China Education Panel Survey, and the Gansu Survey of Children and Families. Analyses reveal a recipro...
Source: Social Science Research - Category: Psychiatry & Psychology Source Type: research
In the wake of the deaths by suicide of two mental health advocates, CNN's Michael Smerconish speaks with Peggy Wehmeyer about living with the shame and stigma of having a loved one die by suicide.
Source: - Health - Category: Consumer Health News Source Type: news
DAVID WALLIAMS is known for his energetic flamboyance and slapstick sense of humour. But this is only one side of his personality. Fans may be surprised to hear that the TV funnyman also grappled with depression.
Source: Daily Express - Health - Category: Consumer Health News Source Type: news
Publication date: October 2019Source: Biomedicine &Pharmacotherapy, Volume 118Author(s): YuZhen Li, XiuHua LiuAbstractApoptosis is an evolutionarily conserved suicide process. It plays critical roles in the development and homeostasis of cardiac tissues. However, excessively stimulated apoptotic activity induced by a myriad of deleterious stimuli can result in too much cardiomyocyte death. The regenerative potential of the adult cardiomyocytes is limited. The cardiomyocyte loss cannot be compensated by efficient cell proliferation. It inevitably leads to various heart diseases. Therefore, the inhibition of cardiomyocyt...
Source: Biomedicine and Pharmacotherapy - Category: Drugs & Pharmacology Source Type: research
Previously observed antidepressant-like effects of caffeine in adolescent rats have been shown in individually housed (IH) rats. Because IH presents a social stressor that may create depressant-like effects in control animals, this study sought to compare the effects of chronic caffeine in IH and pair-housed (PH) adolescent male and female rats. Housing conditions began on postnatal day 24 (P24) and half of the rats were provided caffeine (0.25 g/l) in their drinking water beginning on P28. Open-field behavior was assessed on P42, a light/dark test was conducted on P43, and a forced swim test was conducted on P44–P45...
Source: Behavioural Pharmacology - Category: Drugs & Pharmacology Tags: Research Reports Source Type: research
Many people suffer from a major depressive disorder, and chronic pain conditions are often associated with depressive symptoms. Neurotropin, an extract from the inflamed skin of rabbits inoculated with vaccinia virus, has been used for pain relief. Decrease of brain-derived neurotrophic factor (BDNF) in the brain is one of the proposed mechanisms for the major depressive disorders, and Neurotropin has been reported to restore the decreased BDNF in the hippocampus. In this experiment, we examined whether Neurotropin had an antidepressant-like effect in a model of fibromyalgia and whether BDNF in the brain was altered after ...
Source: Behavioural Pharmacology - Category: Drugs & Pharmacology Tags: Research Reports Source Type: research
More News: Biology | Brain | Depression | Genetics | Neurology