Tailoring of Au-TiO2 Nanoparticles conjugated with Doxorubicin for their Synergistic Response and Photodynamic Therapy Applications

Publication date: Available online 16 August 2019Source: Journal of Photochemistry and Photobiology A: ChemistryAuthor(s): M. Waseem Akram, Fazal Raziq, Muhammad Fakhar-e-Alam, Muhammad Hammad Aziz, K.S. Alimgeer, M. Atif, M. Amir, Atif Hanif, W. Aslam FarooqAbstractAu-TiO2 nanoparticles conjugated with doxorubicin (DOX) were synthesized for new photodynamic therapy (PDT) plans and for comprehensive and reliable treatment of breast cancer-related acute diseases. The anatase/rutile phase junction effect combined with plasmonic resonance is amplified in Au- TiO2 conjugated with DOX, leading to a synergistic response of PDT. Au-TiO2 plays a key role in photocatalytic activity via the development of a new energy bandgap in the composite. The capsules of Au-TiO2@DOX can readily diffuse into the tumor’s vicinity because of the acidic environment of the tissue core, where DOX acts as a photosensitizing agent when Au-TiO2 is excited by an optimal dose of ultraviolet (UV) light, and it demonstrated that a cancer-cell viability loss of up to 70%. This was confirmed by various analytic techniques: reactive oxygen species (ROS) fluorescence, cell mortality assay, MTT assay, apoptosis detection assay, and membrane integrity assay. Furthermore, empirical modeling was performed for statistical analysis of biotoxicity towards the breast cancer cell line. The results acclaim the targeted photokilling efficacy of Au-TiO2@DOX nanocomposites for breast malignancy treatment, as well as in vivo ...
Source: Journal of Photochemistry and Photobiology A: Chemistry - Category: Chemistry Source Type: research