Cobalt bis-dicarbollide and its ammonium derivatives are effective antimicrobial and antibiofilm agents

Publication date: Available online 17 August 2019Source: Journal of Organometallic ChemistryAuthor(s): Eva Vaňková, Kristýna Lokočová, Olga Maťátková, Ivana Křížová, Jan Masák, Bohumír Grüner, Pavel Kaule, Jan Čermák, Václav ŠíchaAbstractThe failure of currently used antibiotics in infections treatment caused by still often emerging microbial resistance is one of the most important worldwide research items. Based on the proved biological activities of cobalt bis-dicarbollide anion [1]- and its derivatives in many medical fields, we tested the sodium salt of cobalt bis-dicarbollide Na[1], and its two ammonium derivatives 8-NH3-[1], 8- PhNH2-[1] to confirm their antimicrobial and, to the best of our knowledge, to demonstrate as first their antibiofilm action. The representatives of the opportunistic pathogens of gram-positive (Staphylococcus spp. and Enterococcus faecalis) and gram-negative bacteria (Pseudomonas aeruginosa and Escherichia coli), yeast Candida parapsilosis and filamentous fungus Trichosporon cutaneum were chosen. Antimicrobial activity was determined by minimum inhibitory concentrations (MIC80) and antibiofilm activity determination was based on MTT assay, crystal violet staining and visualization by microscopic methods. Cytotoxicity against human embryonic kidney cells HEK 293T was measured using resazurin assay. Antimicrobial activity of Na[1], 8-NH3-[1], 8-Ph...
Source: Journal of Organometallic Chemistry - Category: Chemistry Source Type: research

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Source: Chemistry and Biodiversity - Category: Biochemistry Authors: Tags: Chem Biodivers Source Type: research
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Source: Annals of Agricultural and Environmental Medicine : AAEM - Category: Environmental Health Authors: Tags: Ann Agric Environ Med Source Type: research
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Source: Gastroenterology Research and Practice - Category: Gastroenterology Tags: Gastroenterol Res Pract Source Type: research
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Source: Materials Today: Proceedings - Category: Materials Science Source Type: research
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Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
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Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
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Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
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Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
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Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
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