Evaluation of cellular uptake mechanisms for AuNP-collagen-Avemar nanocarrier on transformed and non-transformed cell lines

In this study, we fabricate the Ave-deposited AuNP-collagen nanocarrier (AuNP-Col-Ave) and investigate their endocytic mechanisms in transformed SCC oral cancer cells and non-transformed BAEC and HSF cell lines. By using DLS assay, Ave-deposited AuNP-Col have shown a particle size of 303 ± 35.2 nm. Both UV–vis absorption assay and FTIR spectrum analysis were also demonstrated that the Ave conjugated onto AuNP-Col. Further, both MTT assay and Calcein AM assay were revealed that AuNP-Col-Ave induced a significant cytotoxicity in cancerous SCC cells and showed nontoxicity and biocompatibility for non-transformed BAEC and HFS cells. In addition, AuNP-Col-Ave has showed an excellent uptake capacity in all these cell lines as compared to AuNP-Col group. Further uptake mechanisms analysis demonstrated that the macropinocytosis seems to be the favorite endocytic mechanism during AuNP-Col-Ave internalized into these transformed and non-transformed cell lines. Altogether, this study is first validating the endocytic mechanisms of AuNP-Col-Ave in transformed and non-transformed cell lines. Our findings will provide a novel insight for endocytic mechanisms of cellular uptake nutraceuticals during nutrition therapy and cancer prevention.Graphical abstractSchematic diagram illustrated that AuNP-Col-Ave-mediated cellular uptake mechanisms and cell fates in both transform and non-transformed cell lines.
Source: Colloids and Surfaces A: Physicochemical and Engineering Aspects - Category: Chemistry Source Type: research