Ripk3 licenced protection against microbial infection in the absence of Caspase1-11 inflammasome

Publication date: Available online 17 August 2019Source: Microbes and InfectionAuthor(s): Bojan Shutinoski, Rajen Patel, Julianna J. Tomlinson, Michael G. Schlossmacher, Subash SadAbstractReceptor interacting protein kinase 3 (Ripk3) is a signal relay protein involved in initiation of programmed cell death (necroptosis) and modulation of inflammasome activation. While caspase 1 and 11 are pro-inflammatory caspases responsible for unleashing inflammation and cell death by enzymatic activation of the executioners of inflammation and cell death (pyroptosis). Upon Salmonella infection, the host mounts a pro-inflammatory response which require Ripk3 and Caspase1/11. Here we show that bone marrow derived macrophages with combined deficiency of Ripk3 and Casp1/11 are highly resistant to Salmonella induced cell death, and that these macrophages show an anti-inflammatory cytokine profile. We confirm what was previously known that mice deficient in Casp1/11 have impaired ability to control Salmonella burden, and that the absence of Ripk3 alone does not influence the innate immune responses to Salmonella infection. However, we describe a synergistic role of Ripk3 and Casp1/11 in regulating Salmonella in vivo burden and that Ripk3-dependent host protection in the absence of Casp1/11 is evident during infection by sifA-expressing Salmonella. In summary, we show that the Ripk3 protection to Salmonella infection is obscured by presence of Caspase 1//11 and that the Ripk3-dependent protectio...
Source: Microbes and Infection - Category: Infectious Diseases Source Type: research

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Publication date: Available online 17 September 2019Source: Microbial PathogenesisAuthor(s): Pingping Jiang, Wentao Yang, Yubei Jin, Haibin Huang, Chunwei Shi, Yanlong Jiang, Jianzhong Wang, Yuanhuan Kang, Guilian Yang, Chunfeng WangAbstractLactobacillus reuteri, a typical intestinal symbiotic bacterium, plays an important role in maintaining intestinal flora stability and host health. However, the effect of Lactobacillus reuteri on peritoneal macrophages has not been thoroughly studied. Our study indicated that Lactobacillus reuteri could activate macrophages and that macrophages treated with Lactobacillus reuteri have an...
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research
Scientists from the United Arab Emirates University analysed studies that found injecting cancer-ridden mice with Salmonella causes the pathogen to accumulate in tumours.
Source: the Mail online | Health - Category: Consumer Health News Source Type: news
Contributors : Kelsi O West ; Robert O Watson ; Kristin L.PatrickSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusPurpose: The goals of this study are to compare hnRNP M shRNA knockdown macrophages to scramble shRNA control macrophages in uninfected cells and Salmonella Typhimurium-infecetd cells to unbiasly look at gene expression changes that are regulated by the splicing factor, hnRNP M during resting state and during an innate immune response.Methods: Macrophage mRNA profiles of uninfected and Salmonella Typhimurium-infected hnRNP M knockdown cells lines and SCR shRNA control RAW26...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research
Salmonella infection is a serious concern in poultry farming because of its impact on both economic loss and human health. Chicks aged 20 days or less are extremely vulnerable to Salmonella pullorum (SP), which c...
Source: Genetics Selection Evolution - Category: Genetics & Stem Cells Authors: Tags: Research Article Source Type: research
ConclusionThe features of onset, epidemiological characteristics and molecular subtyping of Salmonella were conducive to clinical diagnosis, rational use of antibiotics and improved therapeutic efficacy.
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Foodborne Pathogens and Disease, Ahead of Print.
Source: Foodborne Pathogens and Disease - Category: Food Science Authors: Source Type: research
Foodborne Pathogens and Disease, Ahead of Print.
Source: Foodborne Pathogens and Disease - Category: Food Science Authors: Source Type: research
The role of illicit THC vape carts in the lung injury outbreak --which has now caused 7 deaths--continues to become clear. Yesterday, Dallas County reported14 new cases ranging in age from 16 to 44 with a median age of 19, all of whom were hospitalized with severe respiratory distress. Although the news headline mentions only that these cases were associated with " vaping, " if you read down to the 11th paragraph, you find out that" Ninety percent of the Dallas County cases reported vaping THC products. "The CDC has continually downplayed the role of THC vape carts in the outbreak and even today, contin...
Source: The Rest of the Story: Tobacco News Analysis and Commentary - Category: Addiction Source Type: blogs
In bacteria, a primary σ-factor associates with the core RNA polymerase (RNAP) to control most transcription initiation, while alternative σ-factors are used to coordinate expression of additional regulons in response to environmental conditions. Many alternative σ-factors are negatively regulated by anti–σ-factors. In Escherichia coli, Salmonella enterica, and many other γ-proteobacteria,...
Source: Proceedings of the National Academy of Sciences - Category: Science Authors: Tags: Biological Sciences Source Type: research
Abstract As a rapid and facile means for foodborne bacteria detection in situ, lateral flow immunoassay (LFA) still has intrinsic limitations in the construction of the existing sandwich LFA format, e.g. screening difficulties of paired antibodies (Abs), poor stability of Ab probe, etc. Here, combined the strong affinity of antibiotic with the superior specificity of antibody molecules, a novel and robust LFA based on a dual recognition strategy and magnetic separation was designed to achieve specific and sensitive determination of Salmonella enteritidis (S. enteritidis). In this work, ampicillin (Amp), a broad-sp...
Source: Talanta - Category: Chemistry Authors: Tags: Talanta Source Type: research
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