Metabolic Engineering of Bacillus subtilis for High ‐Titer Production of Menaquinone‐7

This study constructedBacillus subtilis strains for high ‐titer production of MK‐7 through metabolic engineering approaches. InB. subtilis, MK ‐7 biosynthesis was categorized into five modules: glycerol dissociation pathway, shikimate pathway, pyrimidine metabolic pathway, methylerythritol phosphate pathway, and MK‐7 pathway. Overexpression of GlpK and GlpD (glycerol dissociation pathway) led to a ~10% increase in the MK‐7 titer. Del etion of the genesmgsA andaraM increased the MK ‐7 production by 15%. Furthermore, overexpression of AroGD146N (shikimate pathway), PyrGE156K (pyrimidine metabolic pathway), HepS (methylerythritol phosphate pathway) and VHb could also increase the MK ‐7 titer. Finally we obtained a recombinant strain BSMK_11 with simultaneous overexpressing the genesglpK,glpD,aroGfbr,pyrGfbr,hepS,vgb, and knockouting the genesmgsA andaraM, and the MK ‐7 titer reached 281.4 ± 5.0 mg/L (i.e., 12.0 mg/g DCW) in a 5 L fermenter.This article is protected by copyright. All rights reserved.
Source: AIChE Journal - Category: Science Authors: Tags: BIOMOLECULAR ENGINEERING, BIOENGINEERING, BIOCHEMICALS, BIOFUELS, AND FOOD Source Type: research