Inhibitory activity of Scorzonera latifolia and its components on enzymes connected with healing process

Publication date: Available online 17 August 2019Source: Journal of EthnopharmacologyAuthor(s): Esra Küpeli Akkol, Karel Šmejkal, Ekin Kurtul, Mert Ilhan, Fatma Tuğçe Güragac, Gülçin Saltan Çitoğlu, Özlem Bahadır Acıkara, Josef Cvačka, Miloš BuděšínskýAbstractEthnopharmacological relevanceScorzonera latifolia (Fisch. & Mey.) DC. (Asteraceae)grows naturally in Eastern Anatolia, northeastern Iran, and Caucasus. Latex of S. latifolia roots is used in Turkish folk medicine for its analgesic effects, externally to cure infertility in women, and internally as an antihelmintic. The milk obtained from the stem of the Scorzonera species is used for wound healing activity. Antinociceptive, anti-inflammatory, wound-healing, antioxidant, and antimicrobial activities have previously been reported for S. latifolia.Aim of the study: A methanol extract of the aerial parts of Scorzonera latifolia (Fisch. & Mey.) DC. that had been shown to possess wound-healing activity, was used to elucidate the possible mechanism of the wound-healing activity and to identify the compound(s) responsible for the effect by means of bioassay-guided fractionation.Materials and methodsThe wound-healing activity potential of methanol extract of S. latifolia was detected by evaluating the inhibitory activity on the collagenase, hyaluronidase and elastase, which play important roles in the wound-healing process. Succesive fractionation of the methanol extract using petroleum ether, chloroform, ethyl...
Source: Journal of Ethnopharmacology - Category: Drugs & Pharmacology Source Type: research