Species diversity of faecal microbial flora in Canis lupus familiaris with canine parvovirus disease

Publication date: Available online 16 August 2019Source: Veterinary MicrobiologyAuthor(s): Wang Bi, Xiao-Long WangAbstractParvovirus disease is a highly contagious infectious disease in dogs, which is often accompanied by acute hemorrhagic enteritis and other symptoms, and leads to changes in the faecal microbial flora. To explore these changes in the fecal microbial flora in infected dogs, we used real-time PCR to detect the viral copy numbers in dogs diagnosed with the disease, and measured their blood indices, blood coagulation time, serum calcium, phosphorus, and other biochemical indices. We also determined the species and abundances of the faecal microbial flora of the sick dogs with metagenomic sequencing, and compared them with those of healthy dogs, the viral copy numbers in the dogs were similar, and the blood indices, blood coagulation time, and serum calcium, phosphorus, and other biochemical indices were also consistent. Metagenomic methods revealed relatively more Escherichia, Lachnoclostridium, (Ruminococcus) gnavus group, and uncultured_bacterium_f_lachnospiraceae in the infected dogs, whereas the abundance of Collinsella was relatively reduced. Alloprevotella and Sutterella were not present among the fecal microorganisms of the infected dogs. The relative abundances of Collinsella were significantly reduced in the infected dogs, whereas those of Romboutsia, Erysipelatoclostridium, Anaerotruncus, and Blautia were significantly increased. The functional analysi...
Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research