Evaluation of the BD Phoenix100 System and Colistin Broth Disk Elution Method for Antimicrobial Susceptibility Testing of Colistin Against Gram-negative Bacteria.

[Evaluation of the BD Phoenix100 System and Colistin Broth Disk Elution Method for Antimicrobial Susceptibility Testing of Colistin Against Gram-negative Bacteria]. Mikrobiyol Bul. 2019 Jul;53(3):254-261 Authors: Koyuncu Özyurt Ö, Özhak B, Öğünç D, Yıldız E, Çolak D, Günseren F, Öngüt G Abstract Infections with multidrug resistant gram-negative bacteria is a growing problem especially in health care settings. Colistin is one of the last resort antibiotics for such infections in which treatment options are limited. Increasing resistance to colistin is a global problem. Clinical and Laboratory Standards Institute (CLSI) and the European Committee on Antimicrobial Susceptibility Testing (EUCAST) study groups have recommended the ISO-standard broth microdilution method (20776-1) as the reference method for the determination of colistin susceptibility. Since the broth microdilution method is not a practical method, it is rarely used in routine clinical microbiology laboratories, yet simple and accurate phenotypic detection methods for the determination of colistin resistance in routine microbiology laboratories are not precisely defined. The aim of this study was to evaluate BD Phoenix100 (Becton Dickinson, USA) system and colistin broth disk elution method for the detection of in vitro activity of colistin against gram-negative bacteria. A total of 419 gram-negative bacteria, including 199 Klebsiella pn...
Source: Mikrobiyoloji Bulteni - Category: Microbiology Tags: Mikrobiyol Bul Source Type: research

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In conclusion, mcr-1-positive E. coli environmental isolates were mostly characterised by positive for APEC-associated virulence genes. Co-presence of those genes may suggest the existence of common source in animals and/or their associated environments.IMPORTANCE Colistin is considered as a last-line therapeutic option in severe infections due to multidrug-resistant Gram-negative bacteria, in particular carbapenemase-producing Enterobacteriaceae and multidrug-resistant Acinetobacter baumannii Increasing prevalence of mcr genes in diverse Enterobacteriaceae species, mainly E. coli and Klebsiella pneumoniae from humans and ...
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Tags: Appl Environ Microbiol Source Type: research
Discussion: The infection rate in our centre compared to previous studies of comparable patients was matching. Effective management of postoperative infections should be considered, and identified risk factors in this study can help to focus on effective prevention and treatment strategies. PMID: 31467552 [PubMed]
Source: International Journal of Microbiology - Category: Microbiology Tags: Int J Microbiol Source Type: research
ConclusionsMRSA, ESBL E. coli and ESBL Klebsiella spp were significantly more frequently isolated from hospitalized patients with CRBSI.
Source: American Journal of Infection Control - Category: Infectious Diseases Source Type: research
The lack of therapeutic options to treat infections caused by recalcitrant multidrug-resistant pathogens, especially Gram-negative bacteria, is apparent. Therefore, it is imperative to develop new strategies to address the problem of antimicrobial resistance. Repurposing non-antibiotic commercial drugs for antimicrobial therapy presents a viable option. We screened six anticancer drugs for their potential use in antimicrobial therapy. Here, we provide in vitro evidence that suggests feasibility to repurpose the anticancer drug mitomycin C against multidrug-resistant Gram-negative bacteria. We also demonstrated that mitomyc...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
ConclusionsA baumannii can transfer easily from nitrile gloves to fomite compared with other gram-negative bacteria that cause health care–associated infections. These findings support data from previous observational studies that gloves of health care workers can be major routes of A baumannii transmission in clinical settings.
Source: American Journal of Infection Control - Category: Infectious Diseases Source Type: research
Authors: Alavi M, Rai M Abstract INTRODUCTION: In recent years, multidrug-resistance in bacteria has drastically increased and has posed a great threat to the human health. This problem has generated an urgent need to search alternatives for the treatment of MDR bacteria. It has been proved that metal nanoparticles (MNPs) and nanocomposites (MNCs) possess remarkable antimicrobial potential, and hence can be used in alternative therapy. Areas covered: This review is aimed to discuss recent reports on antibacterial activities of MNPs and MNCs against MDR bacteria. Expert opinion: Escherichia coli, Staphylococcus aure...
Source: Expert Review of Anti-Infective Therapy - Category: Infectious Diseases Tags: Expert Rev Anti Infect Ther Source Type: research
Conclusion In conclusion, our systematic study shows two possible roles of porins, the transport of antibiotics and the membrane integrity regulation, which differently affect antibiotic resistance, and the distinct role of each porin. This provides an important insight to understand not only the role of each porin as a functionally important component of the outer membrane, but also its specific role in the survival of the pathogen under the action of antibiotics. Data Availability All datasets generated for this study are included in the manuscript and/or the Supplementary Files. Author Contributions C-RL contribute...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Conclusion: New carbapenem/βLI combinations may be viable alternatives to antimicrobial combination therapy as they displayed high efficacy in vitro and in vivo. Meropenem/Avibactam and Meropenem/Relebactam should be tested on larger sample sizes with different carbapenemases before progressing further in its preclinical development. Introduction Carbapenem resistant Gram-negative bacteria have been gradually increasing in prevalence in recent years. In the United States, the latest CDC Antibiotic Resistance Threat Report indicates that Carbapenem-Resistant Enterobacteriaceae (CREs) are responsible for 9,000 a...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Application of Antimicrobial Peptides of the Innate Immune System in Combination With Conventional Antibiotics—A Novel Way to Combat Antibiotic Resistance? Maria S. Zharkova1, Dmitriy S. Orlov1, Olga Yu. Golubeva2, Oleg B. Chakchir3, Igor E. Eliseev3, Tatyana M. Grinchuk4 and Olga V. Shamova1* 1Laboratory of Design and Synthesis of Biologically Active Peptides, Department of General Pathology and Pathophysiology, FSBSI Institute of Experimental Medicine, Saint Petersburg, Russia 2Laboratory of Nanostructures Research, Institute of Silicate Chemistry of the Russian Academy of Sciences, Saint Petersburg, Russia ...
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research
Conclusion The results of our study demonstrate that robenidine is not suitable as a sole antimicrobial agent for the treatment of Gram-negative pathogen infections due to the lack of activity against the majority of Gram-negative isolates except for A. baumannii and A. calcoaceticus. However, we demonstrated in vitro efficacy against all selected Gram-negative organisms when robenidine was tested in combination with EDTA or PMBN, including against multidrug-resistant strains. Therefore, robenidine may be an appropriate candidate as a component of a combination preparation for the treatment of otitis externa in dogs. This...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
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