Rapid and quantitative detection of viable emetic Bacillus cereus by PMA-qPCR assay in milk

Publication date: Available online 16 August 2019Source: Molecular and Cellular ProbesAuthor(s): Ping Zhou, Guoyang Xie, Taobo Liang, Bei Yu, Zoraida Aguilar, Hengyi XuAbstractEmetic Bacillus cereus is one of the causative agents of foodborne diseases which can cause vomiting-type food poisoning after ingestion of contaminated food. To minimize B. cereus food poisoning, propidium monoazide (PMA) combined with quantitative polymerase chain reaction (qPCR) called PMA-qPCR was applied for detecting viable emetic B. cereus in milk. The cereulide synthetase gene of emetic B. cereus (cesB) was chosen for the primer, and PMA treatment was optimized at 3 μg/mL to inhibit the PCR amplification of DNA from dead cells. Under optimized assay parameters, the limit of detection (LOD) using this method were 102 CFU/mL in both pure culture and in spiked milk matrix. The cycle threshold (Ct) values obtained for this assay was not significantly affected by the presence of non-target bacteria such as E. coli O157:H7 which indicated the high selectivity of the assay for B. cereus. The PMA-qPCR assay used in this study has the potential for sensitive detection of viable emetic B. cereus in milk.
Source: Molecular and Cellular Probes - Category: Molecular Biology Source Type: research

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Publication date: Available online 19 September 2019Source: Microbial PathogenesisAuthor(s): Muhammad Altaf, Muhammad Ijaz, Awais Ghaffar, Abdul Rehman, Muhammad AvaisAbstractNon-steroidal anti-inflammatory drugs (NSAIDs) may exhibit antibacterial activity and have synergistic effects with antibiotics. One way to re-sensitize MRSA to resistant antibiotics is by combining with approved non-antibiotics. The study was intended to explore susceptibility of MRSA to various antibiotics and non-antibiotics (NSAIDs) by micro dilution broth method. MRSA isolates were confirmed by PCR (mecA gene) and in-vitro antibiotic susceptibili...
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research
ConclusionsIn conclusion, it would appear that the most prevalent mcr gene of E. coli from diarrheic weaned piglets in Korea was mcr-3. And, to our best knowledge, this is the first report for coexistence of two plasmid-mediated colistin resistance genes, mcr-1 and mcr-3 in the same isolates (0258, 0491, 0516) from piglets with diarrhea in Korea. Those mcr–positive isolates showed multi-drug resistance, and majority of those encoded Stx2e and F18. This indicates that the risk of inefficient treatment for edema disease in weaned piglets.
Source: Journal of Global Antimicrobial Resistance - Category: Infectious Diseases Source Type: research
Conclusions: Among children with acute gastroenteritis who were younger than 5 years of age, L. reuteri compared with placebo, as an adjunct to rehydration therapy, did not reduce the duration of diarrhea; however, it reduced the duration of hospitalization.
Source: The Pediatric Infectious Disease Journal - Category: Infectious Diseases Tags: Original Studies Source Type: research
Murat Bağcıoğlu1,2†, Martina Fricker1†‡, Sophia Johler2 and Monika Ehling-Schulz1* 1Functional Microbiology, Institute of Microbiology, Department of Pathobiology, University of Veterinary Medicine Vienna, Vienna, Austria 2Institute for Food Safety and Hygiene, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland The Bacillus cereus group comprises genetical closely related species with variable toxigenic characteristics. However, detection and differentiation of the B. cereus group species in routine diagnostics can be difficult, expensive and laborious since current species desi...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Conclusion Starter cultures are an important tool that contributes to ensure the safety of fermented meat products. Indeed, the microorganisms that constitute starter cultures may inhibit or reduce the growth of spoilage and/or pathogenic populations through mechanisms, such as production of certain metabolites or competitive exclusion. Thus, the use of starter cultures may reduce the need for chemical additives, such as nitrites and nitrates. Furthermore, the lower residual levels of nitrates and nitrites detected in fermented meat products inoculated with starter cultures are due to the ability of starters to metabolize...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
This study provides new insight into the characterization of foodborne C. perfringens and highlights the potential of WGS for the investigation of FBOs. Introduction Clostridium perfringens is a Gram-positive, spore-forming, anaerobic, rod-shaped bacterium, known as an important causative agent of foodborne and non-foodborne gastroenteritis (Grass et al., 2013). The ability of this bacterium to form resistant spores contributes to its survival in many environmental niches, including soil, sewage, foods, and the intestinal microbiota of humans and animals (Xiao, 2014; Li et al., 2016). C. perfringens can cause necro...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Publication date: March 2018 Source:Microbial Pathogenesis, Volume 116 Author(s): Marzie Yeganegi, Farideh Tabatabaei Yazdi, Seyed Ali Mortazavi, Javad Asili, Behrooz Alizadeh Behbahani, Adel Beigbabaei The aerial parts of Equisetum telmateia have been used as a source of biologically active compounds to treat inflammatory, diarrhea, stomach-ache, eczema and mouth infections in traditional medicine. The aim of this work is to evaluate the extraction yield, chemical compositions, antioxidant activity and antimicrobial activity of E. telmateia extracts on Staphylococcus aureus, Bacillus cereus, Escherichia coli, Salmonella ...
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research
Publication date: Available online 24 February 2017 Source:Saudi Journal of Biological Sciences Author(s): Ashraf A. Mostafa, Abdulaziz A. Al-Askar, Khalid S. Almaary, Turki M. Dawoud, Essam N. Sholkamy, Marwah M. Bakri Prevention of food spoilage and food poisoning pathogens is usually achieved by use of chemical preservatives which have negative impacts including: human health hazards of the chemical applications, chemical residues in food & feed chains and acquisition of microbial resistance to the used chemicals. Because of such concerns, the necessity to find a potentially effective, healthy safer and natural alt...
Source: Saudi Journal of Biological Sciences - Category: Biology Source Type: research
Authors: Lixandru BE, Drăcea NO, Dragomirescu CC, Drăgulescu EC, Coldea IL, Anton L, Dobre E, Rovinaru C, Codiţă I Abstract The currative properties of aromatic and medicinal plants have been recognized since ancient times and, more recently, the antimicrobial activity of plant essential oils has been used in several applications, including food preservation. The purpose of this study was to create directly comparable, quantitative data on the antimicrobial activity of some plant essential oils prepared in the National Institute of Research-Development for Chemistry and Petrochemistry, Bucharest to be used for ...
Source: Roumanian Archives of Microbiology and Immunology - Category: Microbiology Tags: Roum Arch Microbiol Immunol Source Type: research
Conclusion: Golnar extract showed antibacterial activity against both Gram positive and Gram negative bacteria causing food poisoning. Therefore, the extract can be used for prevention or treatment of foodborne diseases or as preservative in the food industry.The methanol fraction with the highest phenolic and flavonoid content showed the highest antibacterial effect. This indicates that the phenolic and flavonoid compounds in the extract can be responsible for the effect.
Source: BMC Complementary and Alternative Medicine - Category: Complementary Medicine Authors: Source Type: research
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