Preparation and characterization of a highly soluble Aβ1-42 peptide variant

Publication date: Available online 16 August 2019Source: Protein Expression and PurificationAuthor(s): Marcia A. LeVatte, Matthias Lipfert, Carol Ladner-Keay, David S. WishartAbstractAlzheimer's disease (AD) is a progressive neurological disease marked by the accumulation and deposition of misfolded amyloid beta or Abeta (Aβ) peptide. Two species of Aβ peptides are found in amyloid plaques, Aβ1-40 and Aβ1-42, with the latter being the more amyloidogenic of the two. Understanding how and why Aβ peptides misfold, oligomerize and form amyloid plaques requires a detailed understanding of their structure and dynamics. The poor solubility and strong aggregation tendencies of Aβ1-42 has made the isolation and characterization of its different structural isoforms (monomer, dimer, oligomer, amyloid) exceedingly difficult. Furthermore, while synthetic Aβ1-42 peptides (Aβ42syn) are readily available, the cost of isotopically labeled peptide is substantial, making their characterization by NMR spectroscopy cost prohibitive. Here we describe the design, cloning, high-level production, isotopic labeling and biophysical characterization of a modified (solubility-tagged) Aβ1-42 variant that exhibits excellent water solubility and shares similar aggregation properties as wildtype Aβ1-42. Specifically, we attached six lysines (6K) to the C-terminus of native Aβ1-42 to create a more soluble, monomeric form of Aβ1-42 called Aβ42C6K...
Source: Protein Expression and Purification - Category: Biochemistry Source Type: research

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ConclusionsIn conclusion, it would appear that the most prevalent mcr gene of E. coli from diarrheic weaned piglets in Korea was mcr-3. And, to our best knowledge, this is the first report for coexistence of two plasmid-mediated colistin resistance genes, mcr-1 and mcr-3 in the same isolates (0258, 0491, 0516) from piglets with diarrhea in Korea. Those mcr–positive isolates showed multi-drug resistance, and majority of those encoded Stx2e and F18. This indicates that the risk of inefficient treatment for edema disease in weaned piglets.
Source: Journal of Global Antimicrobial Resistance - Category: Infectious Diseases Source Type: research
Conclusions: Among children with acute gastroenteritis who were younger than 5 years of age, L. reuteri compared with placebo, as an adjunct to rehydration therapy, did not reduce the duration of diarrhea; however, it reduced the duration of hospitalization.
Source: The Pediatric Infectious Disease Journal - Category: Infectious Diseases Tags: Original Studies Source Type: research
In this study, cephalexin (CEX), as an effective antibiotic, was loaded into polycaprolactone (PCL)-gelatin methacryloyl (GelMA) nanofibrous by electrospinning for the development of a new wound dressing. The electrospun nanofibrous possessed continuous and smooth structure with the fiber diameters ranging from 280 to 330 nm. Swelling examination exhibited that the electrospun nanofibrous could take up water by 400–600%. Antibacterial activity of PCL/GelMA loading with CEX had a great inhibition towards both Gram-positive Staphylococcus aureus and negative-bacteria Escherichia coli. The hematoxylin-eosin (H&E) and ...
Source: Materials Letters - Category: Materials Science Source Type: research
In this study, we were constructed a photocatalytic and antibacterial material AgCl/Carbon nanotubes (CNTs)/graphitic carbon nitride (g-C3N4) by deposition-precipitation method. A series of characterizations indicate that AgCl nanoparticles are deposited on CNTs and then dispersed together on g-C3N4 (CN). The photocatalytic properties of the composites were investigated by photodegrading tetracycline under visible light. The AgCl/CNTs/CN nanocomposites exhibit excellent photocatalytic efficiency of 86.44%, which is three times the photocatalytic degradation efficiency of pure CN and AgCl/CN. In addition, the catalytic acti...
Source: Materials Letters - Category: Materials Science Source Type: research
Publication date: 19 September 2019Source: Cell, Volume 179, Issue 1Author(s): Sangjin Kim, Bruno Beltran, Irnov Irnov, Christine Jacobs-WagnerSummaryGenes are often transcribed by multiple RNA polymerases (RNAPs) at densities that can vary widely across genes and environmental conditions. Here, we provide in vitro and in vivo evidence for a built-in mechanism by which co-transcribing RNAPs display either collaborative or antagonistic dynamics over long distances (>2 kb) through transcription-induced DNA supercoiling. In Escherichia coli, when the promoter is active, co-transcribing RNAPs translocate faster th...
Source: Cell - Category: Cytology Source Type: research
Publication date: Available online 19 September 2019Source: Journal of Food EngineeringAuthor(s): Zahra Moaddabdoost Baboli, Leonard Williams, Guibing ChenAbstractJuices are usually thermally pasteurized, but high temperatures negatively affect their nutritional and sensory qualities. For this reason, nonthermal pasteurization techniques with little or no heat applied have been developed to minimize the quality losses. Among them, high-intensity ultrasound has been considered as a promising technique for juice pasteurization. Despite the extensive studies, a long pasteurization time is needed to achieve the required 5-log ...
Source: Journal of Food Engineering - Category: Food Science Source Type: research
Authors: Okai M, Aoki H, Ishida M, Urano N Abstract We investigated the midstream bottom of the Tama River, which flows through Tokyo, to evaluate the occurrence and degree of antibiotic-resistant fecal coliforms including multidrug-resistant fecal coliforms. The genera Klebsiella and Escherichia were the major isolates among the fecal coliforms. For the genus Klebsiella, the highest antibiotic resistance was observed for ampicillin (100%) , followed by kanamycin, tetracycline, cefotaxime, and cefoxitin. The highest resistance to E. coli was found for kanamycin (44.4%) , followed by ampicillin, tetracycline, chlora...
Source: Biocontrol Science - Category: Microbiology Tags: Biocontrol Sci Source Type: research
This study cloned a new crustin from Pacific white shrimp Litopenaeus vannamei, which we designated as LvCrustinB, using rapid amplification of cDNA ends (RACE). The full-length cDNA of LvCrustinB is 751 bp with an open reading frame (ORF) of 591 bp encoding a peptide of 196 amino acids that includes a putative signal sequence. LvCrustinB is a type II crustin that has a glycine-rich region and a single whey acidic protein domain (WAP) domain. The mRNA transcript of LvCrustinB was detected in all examined tissues and was found to be most abundantly expressed in the epithelium and muscle. The expression of LvCrustinB in hemo...
Source: Fish and Shellfish Immunology - Category: Biology Source Type: research
CONCLUSION: We believe that the detection of microorganisms presenting genetic elements in environments such as water is necessary for the prevention and control of their dissemination with potential to infect humans and other animals in eventual contact with these environments. PMID: 31529856 [PubMed - in process]
Source: Biomedica : Revista del Instituto Nacional de Salud - Category: International Medicine & Public Health Tags: Biomedica Source Type: research
CONCLUSIONS: The high frequency of multidrug-resistant ESBL-producing isolates should alert the regional health authorities to take measures to reduce the risk of outbreaks caused by these types of bacteria in the community. PMID: 31529852 [PubMed - in process]
Source: Biomedica : Revista del Instituto Nacional de Salud - Category: International Medicine & Public Health Tags: Biomedica Source Type: research
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