Uptake of Intact Copper Oxide Nanoparticles Causes Acute Toxicity in Cultured Glial Cells.
Uptake of Intact Copper Oxide Nanoparticles Causes Acute Toxicity in Cultured Glial Cells. Neurochem Res. 2019 Aug 14;: Authors: Joshi A, Thiel K, Jog K, Dringen R Abstract Copper oxide nanoparticles (CuO-NPs) dispersions are known for their high cell toxic potential but contaminating copper ions in such dispersions are a major hurdle in the investigation of specific nanoparticle-mediated toxicity. In order to distinguish between the adverse effects exhibited by CuO-NPs and/or by contaminating ionic copper, the membrane-impermeable copper chelator bathocuproine disulfonate (BCS) was added in a low molar ratio (20% of the total copper applied) in order to chelate the copper ions that had been released extracellularly from the CuO-NPs before or during the incubation. Physicochemical characterization of synthesized CuO-NPs revealed that the presence of this low concentration of BCS did not alter the size or zeta potential of the CuO-NPs. Application of CuO-NPs to C6 glioma cells and primary astrocytes induced a concentration- and temperature-dependent copper accumulation which was accompanied by a severe loss in cell viability. The adverse consequences of the CuO-NP application were not affected by the presence of 20% BCS, while the copper accumulation and cell toxicity observed after application of ionic copper were significantly lowered in the presence of BCS. These results demonstrate that for the experimental conditions applied the adverse consequences ...
Publication date: Available online 19 November 2019Source: Journal of Theoretical BiologyAuthor(s): Martina Conte, Luca Gerardo-Giorda, Maria GroppiAbstractA multiscale mathematical model for glioma cell migration and proliferation is proposed, taking into account a possible therapeutic approach. Starting with the description of processes occurring at the subcellular level, the equation for the mesoscopic level is formulated and a macroscopic model is derived, via parabolic limit and Hilbert expansions in the moment equations. After the model set up and the study of the well-posedness of this macroscopic setting, we invest...
CONCLUSIONS: Our studies demonstrated that GLO1 was highly expressed in glioma tissues and significantly correlated with the poor prognosis of glioma patients. It indicated that GLO1 might serve as a new prognostic predictor and therapeutic target for glioma treatment. PMID: 31738028 [PubMed - as supplied by publisher]
We describe the morphologic features of a diagnostically challenging intramedullary spinal cord tumor masquerading as a high grade lesion due its cellular composition and discuss its differentials. The report also emphasizes the role of already established and recently introduced immunohistochemical markers and other ancillary techniques as useful adjuncts in the diagnosis.
In this study, we demonstrated that high expression of miR-9-5p and low expression of forkhead box P2 (FOXP2) were related with better outcome in patients with GBM, and down regulated FOXP2 expression was able to inhibit glioma cells proliferation by cell cycle arrest. Furthermore, we found that FOXP2 was the target protein of miR-9-5p in luciferase assay. The results of this study suggest a novel regulatory mechanism that miR-9-5p can inhibit glioma cells proliferation by downregulating FOXP2.
Authors: Philbrick B, Adamson DC Abstract INTRODUCTION: High grade gliomas (HGG) are extremely aggressive brain malignancies that are usually fatal. Despite maximal resection, chemotherapy and radiation, these tumors inevitably recur and present a poor median overall survival (mOS); hence there is a pressing need for improved treatments.Areas covered:This review assesses DNX-2401 as a treatment of recurrent HGG. Phase I data on efficacy, safety, and tolerability are examined while insights and perspectives on future directions are offered.Expert opinion:This phase I study assessed DNX-2401 in two groups; one group ...
CONCLUSIONS: Results of this study demonstrated that glucose deprivation did not change the expression level of NR3C1 gene but it significantly affected the expression of NR3C2, AHR, NRIP, SGK1, SGK3, and NNT genes in vector-transfected U87 glioma cells in gene specific manner and possibly contributed to the control of glioma growth since the expression of most studied genes in glucose deprivation condition was significantly dependent on the functional activity of IRE1 signaling enzyme. PMID: 31734653 [PubMed - in process]
CONCLUSIONS: Results of this investigation demonstrate that ERN1 knockdown significantly increased the expression of endothelin-1 and its receptors as well as ECE1 genes by different mechanisms and that all studied gene expressions were sensitive to hypoxia. It is possible that hypoxic regulation of the expression of these genes is a result of complex interaction of variable ERN1 related transcription and regulatory factors with HIF1A and possibly contributed to the control of glioma growth. PMID: 31734650 [PubMed - in process]
Conditions: Astrocytoma; Low Grade Glioma Interventions: Drug: Carboplatin; Other: Quality-of-Life Assessment; Other: Questionnaire Administration; Drug: Selumetinib; Drug: Selumetinib Sulfate; Drug: Vincristine; Drug: Vincristine Sulfate Sponsor: National Cancer Institute (NCI) Not yet recruiting
ConclusionTexture analysis of conventional MRI sequences enhanced by ML analysis can accurately predict the IDH1 status of HGG. Adding textural analysis of ADC maps to conventional MRI results in incremental diagnostic performance.
This study suggests that the interplay between sialic acids and Siglecs is a sensitive immune checkpoint axis and may be crucial for Dex-induced dampening of antitumor immunity. The targeting of sialic acid-Siglec glyco-immune checkpoint can be a novel therapeutic method in glioma th erapy.