Ultrasensitive electrochemical detection of Mycobacterium tuberculosis IS6110 fragment using gold nanoparticles decorated fullerene nanoparticles/nitrogen-doped graphene nanosheet as signal tags.
Ultrasensitive electrochemical detection of Mycobacterium tuberculosis IS6110 fragment using gold nanoparticles decorated fullerene nanoparticles/nitrogen-doped graphene nanosheet as signal tags. Anal Chim Acta. 2019 Nov 08;1080:75-83 Authors: Bai L, Chen Y, Liu X, Zhou J, Cao J, Hou L, Guo S Abstract Tuberculosis (TB), caused by Mycobacterium tuberculosis (MTB), remains the top fatal infection continuing to threat public health, and the present detection method for MTB is facing great challenges with the global TB burden. In response to this issue, a novel electrochemical DNA biosensor was developed for detecting the IS6110 fragment within MTB. For the first time, the nanohybrid of gold nanoparticles decorated fullerene nanoparticles/nitrogen-doped graphene nanosheet (Au-nano-C60/NGS) directly served as a new signal tag to generate signal response without additional redox molecules and subsequently labeled with signal probes (SPs) to form tracer label to achieve signal amplification. Additionally, a biotin-avidin system was introduced to immobilize abundant capture probes (CPs), further improving the sensitivity of the proposed biosensor. After a typical sandwich hybridization, the proposed electrochemical DNA biosensor was incubated with tetraoctylammonium bromide (TOAB), which was used as a booster to induce the intrinsic redox activity of the tracer label, resulting in a discriminating current response. The proposed electrochemical DNA biosensor show...
We report the case of a 10-year-old child treated for latent tuberculosis infection (LTBI) with pyrazinamide (PZA) and levofloxacin after contact with a smear-positive multidrug-resistant tuberculosis adult. Over the course of the treatment, the patient developed a drug-induced fulminant hepatitis attributed to the combination of PZA and levofloxacin. This case highlights the hepatotoxicity of the association of second-line anti-TB treatment in children.
Background: The incidence of drug-resistant forms of tuberculosis (DR-TB) and the number of children treated with second-line drugs (SLDs) are increasing. However, limited amount of information is available regarding the use of SLDs in this population. Methods: To describe the treatment of pediatric TB with SLDs and factors associated with use of SLDs in children with and without documented DR-TB, records of pediatric TB patients referred to a center in Italy from 2007 to 2018 were reviewed retrospectively. Results: Of 204 children diagnosed with active TB during the study period, 42 were treated with SLDs because ...
This retrospective study investigated outcomes among lost to follow-up (LTFU) adolescents and young adults (AYAs: 10–24 years of age) with tuberculosis (TB) registered from 2008 to 2014 in Gaborone, using surveillance data. Of 68 LTFU AYAs, 16 repeated treatment; 8 completed and 6 were again LTFU. Of 4 confirmed deaths, 3 had TB/HIV coinfection. Approaches to improve AYA retention in TB care are needed.
Conclusions: The effectiveness of TB management was acceptable. Our routine procedures for the exclusion of TBI appear safe.
This study aimed to assess treatment outcomes of childhood TB in Chókwè District, Mozambique. Methods: A retrospective cohort study of children
[Nyasa Times] Community sputum collection points across Malawi managed to identify at least 1000 Tuberculosis (TB) patients in 2018 alone and helped the sick to access treatment, effectively avoiding transmission which could have lead to 15,000 new TB cases.