Silencing LINC00511 inhibits cell proliferation, migration and EMT via PTEN/AKT/FOXO1 signaling pathway in lung cancer.

Silencing LINC00511 inhibits cell proliferation, migration and EMT via PTEN/AKT/FOXO1 signaling pathway in lung cancer. Biochem Cell Biol. 2019 Aug 15;: Authors: Jiang L, Xie X, Ding F, Mei J, Bi R Abstract Lung cancer is the most common cause of cancer-related death worldwide. Long noncoding RNAs (lncRNAs) are longer than 200 nucleotide transcripts and are not translated into proteins. Increasing evidence has shown that lncRNAs are associated with several biological processes in cancer. However, the roles of LINC00511 in lung cancer progression remain unknown. In the present study, we confirmed that LINC00511 knockdown significantly inhibited cell proliferation and migration in A549, SPCA1 and H460 cells. Western blot results showed that silencing LINC00511 inhibited epithelial-mesenchymal transition (EMT), including decreases in the expression levels of ZEB2, N-cadherin and Vimentin and an increase in the expression level of E-cadherin. Additionally, silencing LINC00511 significantly upregulated PTEN mRNA and protein expression, increased FOXO1 and inactivated AKT. Furthermore, we found that PTEN knockdown reversed the inhibition of cell migration and proliferation induced by LINC00511 siRNA, markedly reduced p-FOXO1 expression and promoted p-AKT expression and EMT in A549 and H460 cells. Therefore, these findings revealed that LINC00511 functions as an oncogene via the PTEN/AKT/FOXO1 signaling pathway in lung cancer, providing a p...
Source: Biochemistry and Cell Biology - Category: Biochemistry Authors: Tags: Biochem Cell Biol Source Type: research