Enhancing coverage in LC-MS-based untargeted metabolomics by a new sample preparation procedure using mixed-mode solid-phase extraction and two derivatizations.

Enhancing coverage in LC-MS-based untargeted metabolomics by a new sample preparation procedure using mixed-mode solid-phase extraction and two derivatizations. Anal Bioanal Chem. 2019 Aug 14;: Authors: Wu Q, Xu Y, Ji H, Wang Y, Zhang Z, Lu H Abstract It is a challenge to expand the metabolome coverage of liquid chromatography (LC)-electrospray ionization (ESI) mass spectrometry (MS) based untargeted metabolomics analysis. The limited coverage is attributed to the weak signal of hydroxyl and carboxyl groups in ESI-MS and the limited capacity of LC separation for metabolites with a wide range of polarities. Here a new sample preparation procedure is proposed to solve these problems. Mixed-mode (reversed-phase and anion-exchange) solid-phase extraction sorbents were used to separate metabolites into hydrophilic amine, hydrophobic amine/alcohol, and organic acid groups. Then, alcohols and carboxylic acids in separated groups were tagged with pyridine with use of two derivatization systems for signal enhancement. Finally, hydrophilic amines were analyzed by LC-MS with a hydrophilic interaction LC column, and the two hydrophobic compound groups were analyzed by LC-MS with a C18 column. From the results for standard samples, the detection limits of the new method are lower than those of the classic solvent extraction-protein precipitation method by 3.3-70 times for five amino acids and by 65-1141 times for five fatty acids. Moreover, the detection limit of thi...
Source: Analytical and Bioanalytical Chemistry - Category: Chemistry Authors: Tags: Anal Bioanal Chem Source Type: research

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Authors: Kwako LE, Patterson J, Salloum IM, Trim RS PMID: 31942280 [PubMed - in process]
Source: Alcohol Research - Category: Addiction Tags: Alcohol Res Source Type: research
Publication date: 15 April 2020Source: Journal of Molecular Structure, Volume 1206Author(s): Seyed Reza Nokhbeh, Mostafa Gholizadeh, Alireza Salimi, Hazel A. SparkesAbstractThis paper presents synthesis and structural characterization of new members of phosphorus-based organic bromides. 1,3-Propanediylbis(triphenylphosphonium) dibromide I and 1,3-propanediylbis(triphenylphosphonium) monotribromide II, as a new brominating agent for double bonds and phenolic rings, were synthesized. 1H NMR, 13C NMR, 31P NMR, FT-IR, single crystal X-ray diffraction crystallography, differential scanning calorimetry, thermogravimetric analysi...
Source: Journal of Molecular Structure - Category: Molecular Biology Source Type: research
Chem. Commun., 2020, Accepted Manuscript DOI: 10.1039/C9CC09103C, CommunicationFranziska K ühn, Satoko Katsuragi, Yasuhiro Oki, Cedric Scholz, Shuji Akai, Harald Gröger In spite of the tremendous success of dynamic kinetic resolutions for a broad range of compound classes, tertiary alcohols and their corresponding esters have still remained as one of the... The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Chem. Commun. latest articles - Category: Chemistry Authors: Source Type: research
Publication date: Available online 17 January 2020Source: Journal of Hazardous MaterialsAuthor(s): Lingli Zhu, Dekui Shen, Kai Hong LuoAbstractVolatile organic compounds (VOCs) have attracted world-wide attention regarding their serious hazards on ecological environment and human health. Industrial processes such as fossil fuel combustion, petrochemicals, painting, coatings, pesticides, plastics, contributed to the large proportion of anthropogenic VOCs emission. Destructive methods (catalysis oxidation and biofiltration) and recovery methods (absorption, adsorption, condensation and membrane separation) have been develope...
Source: Journal of Hazardous Materials - Category: Environmental Health Source Type: research
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Source: Journal of Hazardous Materials - Category: Environmental Health Source Type: research
Brief alcohol interventions are an effective strategy for reducing harmful and risky alcohol use and misuse. Many effective brief alcohol interventions include information and advice about an individual's alcohol use, changing their use, and assistance in ...
Source: SafetyLit - Category: International Medicine & Public Health Tags: Age: Adolescents Source Type: news
Conclusion The periconceptional environment and lifestyle factors modify sperm epigenome. This alteration might be maintained in the zygote and throughout development, thereby leading to the inheritance of newly acquired pathologies. The role of sperm miRNA, not only as innovative markers of fertility issues but also as vectors involved in the inheritance of paternal diseases, appears to be crucial. Overweight and obesity seem to alter sperm miRNA profile, thereby leading to transmission of different miRNA profiles in zygote, with consequences on embryo development. In long term, metabolic disorders have been described in...
Source: Frontiers in Genetics - Category: Genetics & Stem Cells Source Type: research
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Source: Frontiers in Psychiatry - Category: Psychiatry Source Type: research
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Source: Current Obesity Reports - Category: Eating Disorders and Weight Management Source Type: research
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Source: PharmaNutrition - Category: Nutrition Source Type: research
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