The effect of an alternative chromosome 17 probe on fluorescence in situ hybridization for the assessment of HER2 amplification in invasive breast cancer.

The effect of an alternative chromosome 17 probe on fluorescence in situ hybridization for the assessment of HER2 amplification in invasive breast cancer. Exp Ther Med. 2019 Sep;18(3):2095-2103 Authors: Xu Z, Xu P, Fan W, Huang B, Cheng Q, Zhang Z, Wang P, Yu M Abstract Fluorescent in situ hybridization (FISH) is commonly used to determine the ratio of human epidermal growth factor receptor 2 (HER2) to centromere enumeration probe for chromosome 17 (CEP17), which further determines HER2 gene status in breast cancer. However, due to copy number alteration in CEP17, inaccurate diagnoses can occur. The current study was performed to investigate the diagnostic value of an alternative CEP17 reference probe for HER2 status in invasive breast cancer. A higher-order repeat in the centromeric region of chromosome 17 was identified and an alternative probe (SCEP17) was subsequently prepared. Karyotype analysis of peripheral blood was used to detect SCEP17 probe specificity. Using a HER2/CEP17 probe, karyotype analysis revealed two strong green signals at the centromere of chromosome 17 and one weaker signal at the other centromere. However, two strong hybridization signals at the centromere of chromosome 17 were observed when the HER2/SCEP17 probe was used. In the 425 patients with invasive breast cancer, no statistical difference was observed between HER2/SCEP17 and HER2/CEP17 when detecting HER2 gene amplification (P=0.157). However, in terms of copy number, the ...
Source: Experimental and Therapeutic Medicine - Category: General Medicine Tags: Exp Ther Med Source Type: research

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Source: Breast Cancer Research and Treatment - Category: Cancer & Oncology Source Type: research
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Source: Breast Cancer Research and Treatment - Category: Cancer & Oncology Source Type: research
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Source: Breast Cancer Research and Treatment - Category: Cancer & Oncology Source Type: research
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Source: Breast Cancer Research and Treatment - Category: Cancer & Oncology Source Type: research
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Source: Breast Cancer Research and Treatment - Category: Cancer & Oncology Source Type: research
ConclusionsThere is modest correlation in continuous gene expression, as measured by the RS and single-gene results for ER, PR, and HER2 between paired primary tumors and synchronous nodal metastases. RS testing for ER+ breast cancer should continue to be based on analysis of primary tumors.
Source: Breast Cancer Research and Treatment - Category: Cancer & Oncology Source Type: research
Roche today announced the phase III FeDeriCa study met its primary endpoint. The study showed a new investigational fixed-dose combination (FDC) of Perjeta ® (pertuzumab) and Herceptin® (trastuzumab), administered by subcutaneous (SC) injection in combination with intravenous (IV) chemotherapy, demonstrated non-inferior levels of Perjeta in the blood (pharmacokinetics) compared to standard IV infusion of Perjeta plus Herceptin and chemotherapy in peo ple with HER2-positive early breast cancer (eBC).
Source: Roche Media News - Category: Pharmaceuticals Source Type: news
Hoskin Many dietary flavonoids possess anti-cancer activities. Here, the effect of apple peel flavonoid fraction 4 (AF4) on the growth of triple-negative (MDA-MB-231, MDA-MB-468), estrogen receptor-positive (MCF-7), and HER2-positive (SKBR3) breast cancer cells was determined and compared with the effect of AF4 on normal mammary epithelial cells and dermal fibroblasts. AF4 inhibited breast cancer cell growth in monolayer cultures, as well as the growth of MCF-7 spheroids, without substantially affecting the viability of non-malignant cells. A sub-cytotoxic concentration of AF4 suppressed the proliferation of MDA-MB-23...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research
Conditions:   Breast Cancer Female;   HER2-positive Breast Cancer;   Hormone Receptor Positive Malignant Neoplasm of Breast;   Metastatic Breast Cancer;   Breast Diseases;   Hormone Receptor Positive Tumor Interventions:   Drug: Pyrotinib;   Drug: Trastuzumab;   Drug: Aromatase Inhibitors Sponsor:   Fuzhou General Hospital Not yet recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
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Source: The American Journal of Surgical Pathology - Category: Pathology Tags: Original Articles Source Type: research
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