Diurnal expression of ABC and SLC transporters in jejunum is modulated by adrenalectomy

Publication date: Available online 15 August 2019Source: Comparative Biochemistry and Physiology Part C: Toxicology & PharmacologyAuthor(s): Karla Vagnerová, Peter Ergang, Matúš Soták, Kateřina Balounová, Pavlína Kvapilová, Martin Vodička, Jiří PáchaAbstractThe circadian clock system drives many physiological processes, including plasma concentration of glucocorticoids and epithelial transport of some ions and nutrients. As glucocorticoids entrain the circadian rhythms in various peripheral organs, we examined whether adrenalectomy affects the expression and circadian rhythmicity of intestinal transporters of the solute carrier (SLC) and ATP-binding cassette (ABC) families, which participate in intestinal barriers for absorption of nutrients, nonnutrients and oral drugs. The rat jejunum showed rhythmic circadian profiles of Sglt1, Pept1, Nhe3, Mdr1 and Mrp2 but not Mct1, Oct1, Octn1, Oatp1, Cnt1 and Bcrp. With the exception of Pept1 and Mct1, adrenalectomy decreased the expression of all rhythmic and arrhythmic transporters including the amplitude of Sglt1 and Nhe3 rhythms but minimally affected the phases of rhythmic transporters except of Nhe3. Similarly, adrenalectomy downregulated the expression of rhythmic (Pparα, Hlf, Pgc1α) and arrhythmic (Hnf1β, Hnf4α) transcription factors, which are known to regulate the expression of transporters. We conclude that endogenous corticosteroids have a profound effect on the expression of intestinal SLC and ABC transport...
Source: Comparative Biochemistry and Physiology Part C: Toxicology and Pharmacology - Category: Toxicology Source Type: research