Evaluation of physiological changes induced by the fluoroquinolone antibiotic ciprofloxacin in the freshwater macrophyte species Lemna minor and Lemna gibba

In this study we selected two species of aquatic macrophytes, Lemna minor and Lemna gibba, which are standard plant species inscribed in ecotoxicological testing guidelines. In this work we characterized the toxic effects of the quinolone antibiotic ciprofloxacin (in levels of 0.005, 0.013, 0.031, 0.078, and 0.195 mg/L), focusing on its potential toxicity towards photosynthetic mechanisms, and pro-oxidant effects. These objectives were attained by measuring the concentrations of chlorophyll a and b, and carotenoids levels. The determination of the quantum yield allowed assessing the effects of ciprofloxacin on the photochemical efficiency of the Photosystem II (PSII). The pro-oxidant effects induced by ciprofloxacin were evaluated by measuring oxidative stress biomarkers, such as catalase activity, and also by determining lipoperoxidation levels. The obtained results showed no differences in terms of the content of both chlorophylls a and b, or any change in the photochemical efficiency of the PSII; however, the global carotenoids content of L. gibba were significantly decreased. The activity of the anti-oxidant enzyme catalase was also significantly increased in L. minor. L. gibba showed a decrease in lipid peroxidation levels, but only for the two lowest concentrations of ciprofloxacin. The global set of data shows the activation of the anti-oxidant defensive system of both plant species, a response that was likely activated by the pro-oxidant character of ciprofloxacin. ...
Source: Environmental Toxicology and Pharmacology - Category: Environmental Health Source Type: research