Timing of Sacral Neurostimulation is Important for Increasing Bladder Capacity in the Anesthetized Rat.

Timing of Sacral Neurostimulation is Important for Increasing Bladder Capacity in the Anesthetized Rat. Am J Physiol Renal Physiol. 2019 Aug 14;: Authors: Potts BA, Degoski DJ, Brooks JM, Peterson AC, Nelson DE, Brink TS, Fraser MO Abstract We assessed the effects of limited application of SNS during bladder filling on bladder capacity utilizing our previously published sacral neurostimulation (SNS) model in rats. Female Sprague-Dawley rats (n=24) were urethane-anesthetized (s.c. 1.2 g/kg) and implanted with jugular venous and transvesical bladder catheters. The L6/S1 nerve trunks were isolated bilaterally and two electrodes were placed on each exposed nerve. True bladder capacity (TBC) was determined using stable single-fill cystometrograms. In the first series of experiments, SNS was applied at the onset of bladder filling for 25%, 50%, 75%, and 100% of the previous control filling cycle duration (n=10). In the second series of experiments, SNS was applied during the first, second, third, and fourth 25% and the first and second 50% of control fill. In the first series, a significant increase in TBC was observed only when SNS was applied for 75% or 100% of control fill duration (30 and 35%, resp., p<0.05). In the second series, significant increases in TBC only occurred during the fourth 25% and second 50% periods (32 and 43%, resp., p<0.001). Results from the second series also revealed an increase in subsequent single-fill b...
Source: American Journal of Physiology. Renal Physiology - Category: Physiology Authors: Tags: Am J Physiol Renal Physiol Source Type: research