Utility of targeted, amplicon-based deep sequencing to detect resistance to first-line tuberculosis drugs in Botswana.

Utility of targeted, amplicon-based deep sequencing to detect resistance to first-line tuberculosis drugs in Botswana. Antimicrob Agents Chemother. 2019 Aug 12;: Authors: Wang Q, Modongo C, Allender C, Engelthaler DM, Warren RM, Zetola NM, Shin SS Abstract Multidrug-resistant tuberculosis is an alarming threat and targeted deep sequencing (DS) may be an effective method for rapid identification of drug-resistant profiles, including detection of heteroresistance. We evaluated sensitivity and specificity of targeted DS vs. phenotypic drug susceptibility testing (pDST) among patients starting first-line anti-TB therapy in Botswana. Overall, we found high concordance between DS and pDST. Lower sensitivity of DS, which targets established high-confidence resistance variants, was observed for detecting isoniazid-resistance among HIV-infected patients. PMID: 31405858 [PubMed - as supplied by publisher]
Source: Antimicrobial Agents and Chemotherapy - Category: Microbiology Authors: Tags: Antimicrob Agents Chemother Source Type: research