Disparate Candida albicans biofilm formation in clinical lipid emulsions due to capric acid mediated inhibition.

Disparate Candida albicans biofilm formation in clinical lipid emulsions due to capric acid mediated inhibition. Antimicrob Agents Chemother. 2019 Aug 12;: Authors: Willems HME, Stultz JS, Coltrane ME, Fortwendel JP, Peters BM Abstract Receipt of parenteral nutrition (PN) remains an independent risk factor for developing catheter-related blood stream infections (CR-BSI) caused by fungi, including the polymorphic fungus Candida albicans that is notoriously adept at forming drug-resistant biofilm structures. Among a variety of macronutrients, PN solutions contain lipid emulsions to supply daily essential fats and are often delivered via central venous catheters (CVC). Therefore, using an in vitro biofilm model system, we sought to determine whether various clinical lipid emulsions differentially impacted biofilm growth in C. albicans We observed that lipid emulsions Intralipid® and Omegaven® both stimulated C. albicans biofilm formation during growth in minimal medium or a macronutrient PN solution. Conversely, Smoflipid® inhibited C. albicans biofilm formation by approximately 50%. Follow up studies revealed that while Smoflipid did not impair C. albicans growth, it did significantly inhibit hypha formation and hyphal elongation. Moreover, growth inhibition could be recapitulated in Intralipid® when supplemented with capric acid-a fatty acid present in Smoflipid® but absent in Intralipid®. Capric acid was also found to dose-depe...
Source: Antimicrobial Agents and Chemotherapy - Category: Microbiology Authors: Tags: Antimicrob Agents Chemother Source Type: research