Skeletal muscle induce recruitment of Ly6C+ macrophages subtype and release inflammatory cytokines 3 days after downhill exercise.

Skeletal muscle induce recruitment of Ly6C+ macrophages subtype and release inflammatory cytokines 3 days after downhill exercise. Am J Physiol Regul Integr Comp Physiol. 2019 Aug 14;: Authors: Minari ALA, Avila F, Oyama LM, Thomatieli-Santos RV Abstract Macrophages are one of the most versatile cells of the immune system, which can express distinct subtypes and functions depending on the physiological challenge. After skeletal muscle damage, inflammatory macrophages subtypes aids muscles to regeneration and are implicate on physical training adaption. Based on this, this study aimed to evaluate two classic mice macrophage subtypes, and some inflammatory cytokines might involve with muscle adaption process after exercise. For this purpose, mice were exposed to an intermittent experimental protocol of downhill exercise (18 bouts of running, each bout of 5 minutes with a 2 minute rest interval, slope -16°) and euthanized before (CTRL), and one (D1), two (D2), and three (D3) days after exercise. After euthanasia, the Triceps brachii (TB) was harvest and submitted to protein extraction, immunostaining, and mononuclear digestion procedures. The muscle size, macrophage accumulation, and cytokines were determined. We observed an increase in Ly6C+ macrophages subtype (p-value ≤ 0.05) in D2 and D3 compared with the control as well as a significant inverse correlation (C.C=-0.52; p-value ≤ 0.05) between Ly6C+ and Ly6C- macrophage subtypes...
Source: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology - Category: Physiology Authors: Tags: Am J Physiol Regul Integr Comp Physiol Source Type: research