Imaging aquatic animal cells and associated pathogens by atomic force microscopy in air.

In this study, we used AFM to image three different types of biological material from shrimp (Litopenaeus vannamei) in air, including hemocytes and two associated pathogens. Without obvious deformations when the cells were imaged in air and in the case for the haemocytes and the cells were fixed as well. The result suggests hydrophobic glass coverslips are a suitable substrate for adhesion of these samples. The method described here can be applied to the preparation of other fragile biological samples from aquatic animals for high-resolution analyses of host-pathogen interactions and other basic physiological processes. PMID: 31407133 [PubMed - as supplied by publisher]

https://www.ncbi.nlm.nih.gov/pubmed/31407133?dopt=Abstract

Source: Biotechnology Letters - August 12, 2019 Category: Biotechnology Authors: Li C, Liang Y, Xu M, Wang H, Wang C, Huang J Tags: Biotechnol Lett Source Type: research