Localization and regulation of polymeric immunoglobulin receptor (PIgR) in healthy and diseased human kidney.

Localization and regulation of polymeric immunoglobulin receptor (PIgR) in healthy and diseased human kidney. Am J Pathol. 2019 Aug 09;: Authors: Krawczyk KM, Nilsson H, Nyström J, Lindgren D, Leandersson K, Swärd K, Johansson ME Abstract The polymeric immunoglobulin receptor (PIgR) constitutes an important part of the immune system by mediating transcytosis of dimeric IgA into mucosal fluids. Although well studied in organs such as the intestine, the regulation and localization of PIgR in human kidney are incompletely characterized. Here, using immunohistochemistry we show that in healthy human kidneys, PIgR is expressed by the progenitor-like tubular scattered cells of the proximal tubules and by parietal epithelial cells of glomeruli. We further show that proximal tubular expression of PIgR becomes widespread during kidney disease, correlating to elevated levels of urinary secretory IgA as determined by ELISA. Urinary secretory IgA levels also correlated to the degree of tubular fibrosis, plasma creatinine, and urea levels. In addition, primary tubular cells were cultured to study the function and regulation of PIgR in vitro. Cellular PIgR expression was induced by conditioned medium from activated human leukocytes, as well as by inflammatory cytokines, whereas transforming growth factor-β1 caused decreased expression. Furthermore, IFNγ increased the transcytosis of dimeric IgA in cultured tubular cells. Finally, a correlation...
Source: The American Journal of Pathology - Category: Pathology Authors: Tags: Am J Pathol Source Type: research