The comparative effect of magnetic activated cell sorting, density gradient centrifugation and swim up on assisted reproduction outcomes, sperm DNA fragmentation, and aneuploidy: A systematic review and meta-analysis

Publication date: Available online 14 August 2019Source: Meta GeneAuthor(s): Yousef Daneshmandpour, Fariba Pashazadeh, Fereshteh Ansari, Hossein Hosseinifard, Mohammad Nouri, Mehdi Yousefi, Ebrahim SakhiniaAbstract:Assisted Reproduction Techniques (ARTs) are commonly used worldwide, and sperm quality has great effect on its successfulness. Different sperm separation techniques have been developed to increase the quality of used sperms in ART. The aim is to determine the effect of sperm separation techniques including Magnetic Activated Cell Sorting (MACS), Density Gradient Centrifugation (DGC) and Swim up (SU) on pregnancy, implantation, miscarriage rates (as the primary outcomes), and also DNA fragmentation and aneuploidy (as the secondary outcomes). PubMed, EMBASE, ProQuest, Cochrane, clinicaltrials.gov, IRCT.ir, and Google Scholar databases were searched. Five RCTs were included to analyze the effects of MACS, DGC, and SU on pregnancy, implantation, and miscarriage rates. Thirty-three studies were included to analyze the effects of mentioned techniques on DNA fragmentation and aneuploidy. Our results indicated that MACS is the best sperm separation technique when compared to DGC and SU and it resulted in a higher pregnancy rate. Also, sperm separation techniques had positive effect on decreasing DNA fragmentation level and aneuploidy in sperm cells.
Source: Meta Gene - Category: Genetics & Stem Cells Source Type: research