Exogenous acquisition of Pseudomonas aeruginosa in intensive care units: a prospective multicentre study, DYNAPYO study.

Publication date: Available online 13 August 2019Source: Journal of Hospital InfectionAuthor(s): Maïder Coppry, Camille Leroyer, Marion Saly, Anne-Gaëlle Venier, Céline Slekovec, Xavier Bertrand, Sylvie Parer, Serge Alfandari, Emmanuelle Cambau, Bruno Megarbane, Christine Lawrence, Bernard Clair, Alain Lepape, Pierre Cassier, Dominique Trivier, Alexandre Boyer, Hélène Boulestreau, Julien Asselineau, Véronique Dubois, Rodolphe ThiébautSummaryPseudomonas aeruginosa remains one of the most common nosocomial pathogens in intensive care units (ICUs). Although exogenous acquisition has been widely documented in outbreaks, its importance is unclear in non-epidemic situations. We aimed to elucidate the role of exogenous origin of P. aeruginosa in ICU patients.We performed a chronological analysis of the acquisition of P. aeruginosa using samples collected in 2009 in DYNAPYO cohort study during which patients and tap water were weekly screened. Molecular relatedness of P. aeruginosa isolates was investigated by pulsed-field gel electrophoresis. Exogenous acquisition was defined as identification of a P. aeruginosa pulsotype previously isolated from another patient or tap water in the ICU.DYNAPYO cohort included 1,808 patients (10,402 samples) and 233 water taps (4,946 samples). Typing of 1,515 isolates from 373 patients and 375 isolates from 81 tap water samples identified 296 pulsotypes. Analysis showed an exogenous acquisition in 170 (45.6%) of 373 patients. The pulsotype ide...
Source: Journal of Hospital Infection - Category: Infectious Diseases Source Type: research