An improved system for constructing marker-free recombinant goatpox viruses to express foreign proteins

In this study, our previous GTPV-generating system was modified by reconstruction of a Loxp-containing transfer plasmid for deleting markers using the Cre/Loxp system. Meanwhile, the previous method was significantly improved by introduction of an immortalized goat testis cell line as a substitute for primary cells. Based on the latest system, a marker-free recombinant GTPV was reconstructed for expressing the EG95 antigen, and showed neither a significant difference in replication kinetics from its parental virus nor a mutation in the foreign sequence during serial 10 passages in vitro.
Source: Research in Veterinary Science - Category: Veterinary Research Source Type: research