Implementation of the Rapid Polymyxin Acinetobacter Test to Detect Colistin-Resistant Acinetobacter baumannii

Microbial Drug Resistance, Ahead of Print.
Source: Microbial Drug Resistance - Category: Microbiology Authors: Source Type: research

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Publication date: Available online 23 January 2020Source: LWTAuthor(s): Xin-Yun Wang, Jing XieAbstractAcinetobacter johnsonii and Pseudomonas fluorescens are specific spoilage microorganisms (SSO) of aquatic products, and their metabolites are effective indicators for analyzing these two SSOs. However, the metabolome of a coculture of A. johnsonii and P. fluorescens is still unclear. The aim of this study was to investigate the metabolomic changes in A. johnsonii, P. fluorescens, and their co-culture by screening for metabolic markers. PCA and PLS-DA revealed that A, P, and AP groups were different from each other, indicat...
Source: LWT Food Science and Technology - Category: Food Science Source Type: research
Green Chem., 2020, Accepted Manuscript DOI: 10.1039/C9GC03478A, PaperAtsushi Usami, Masahito Ishikawa, Katsutoshi Hori Gas-phase bioproduction, in which immobilized biocatalysts are employed and chemical reactions are performed in a gas phase, has attracted researchers ’ attention as a green process. However, there is difficulty in... The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Green Chem. latest articles - Category: Chemistry Authors: Source Type: research
In its native environment of rotting vegetation, the soil nematode Caenorhabditis elegans encounters a range of bacteria. This includes species from the ESKAPE group of pathogens that pose a clinical problem in acquired hospital infections. Here, we investigated three Gram-negative members of the ESKAPE group, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Acinetobacter baumannii. Pathogenicity profiles as measured by time to kill adult C. elegans showed that P. aeruginosa was the most pathogenic, followed by K. pneumoniae, while C. elegans cultured on A. baumannii exhibited the same survival as those on the standard l...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
AbstractAcinetobacter baumannii is well known for causing hospital ‐associated infections due in part to its intrinsic antibiotic resistance as well as its ability to remain viable on surfaces and resist cleaning agents. In a previous publication,A. baumannii strain AB5075 was studied by transposon mutagenesis and 438 essential gene candidates for growth on rich ‐medium were identified. The Seattle Structural Genomics Center for Infectious Disease entered 342 of these candidate essential genes into our pipeline for structure determination, in which 306 were successfully cloned into expression vectors, 192 were detectab...
Source: Protein Science - Category: Biochemistry Authors: Tags: Article Source Type: research
jamin Parkinson We aimed to describe the epidemiology, multi-drug resistance and seasonal distribution of bacteria cultured within 12 months following lower limb orthopaedic surgery in tropical and subtropical Australian hospitals between 2010 and 2017. We collected data from four tropical and two subtropical hospitals. Categorical variables were examined using the Pearson Chi-squared test or Fisher’s Exact test, and continuous variables with the Student t-test or Mann–Whitney U test. A Poisson regression model was used to examine the relationship between season, weather and the incidence of Staphyl...
Source: International Journal of Environmental Research and Public Health - Category: Environmental Health Authors: Tags: Article Source Type: research
This study showed the natural evolution of indigenous fermented meat starter cultures and confirmed a higher suitability of horse and beef meat for nitrate/nitrite free salami production due to their hygienic quality at 30 d. PMID: 31948626 [PubMed - in process]
Source: Food Microbiology - Category: Food Science Authors: Tags: Food Microbiol Source Type: research
Abstract Microbial remediation has the potential to inexpensively yet effectively decontaminate and restore contaminated environments, but the virulence of pathogens and risk of resistance gene transmission by microorganisms during antibiotic removal often limit its implementation. Here, a cloned tetX gene with clear evolutionary history was expressed to explore doxycycline (DOX) degradation and resistance variation during the degradation process. Phylogenetic analysis of tetX genes showed high similarity with those of pathogenic bacteria, such as Riemerella sp. and Acinetobacter sp. Successful tetX expression was...
Source: Ecotoxicology and Environmental Safety - Category: Environmental Health Authors: Tags: Ecotoxicol Environ Saf Source Type: research
Acinetobacter baumannii (A. baumannii) is an important opportunistic pathogen causing serious nosocomial infections, which is considered as the most threatening Gram-negative bacteria (GNB). Outer membrane protei...
Source: Journal of Biomedical Science - Category: Biomedical Science Authors: Tags: Review Source Type: research
Publication date: Available online 15 January 2020Source: Microbiological ResearchAuthor(s): Ashley N. Williams, John StavrinidesAbstractMulti-drug resistant Acinetobacter baumannii and Pseudomonas aeruginosa continue to pose a serious health threat worldwide. Two Pantoea agglomerans strains, 3581 and SN01080, produce an antibiotic effective against these pathogens. To identify the antibiotic biosynthetic gene clusters, independent genetic screens were conducted for each strain using a mini-Tn5 transposon, which resulted in the identification of the same conserved eight-gene cluster. We have named this antibiotic Pantoea N...
Source: Microbiological Research - Category: Infectious Diseases Source Type: research
Authors: Escolà-Vergé L, Los-Arcos I, Almirante B Abstract One of the current priorities of the World Health Organization is multidrug-resistant bacteria, because they are a global problem due to their rapid spread and the difficulty of their treatment. In addition, they are associated with high morbidity, mortality and high economic costs. There are multidrug-resistant bacteria, both Gram-positive and Gram-negative, including Pseudomonas aeruginosa and Acinetobacter baumannii resistant to carbapenems, enterobacteria producing carbapenemases, Staphylococcus aureus resistant to methicillin and/or with ...
Source: Medicina Clinica - Category: General Medicine Tags: Med Clin (Barc) Source Type: research
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