Development of RT-PCR assays for the detection and the resultant phylogenetic analysis of four grapevine vitiviruses based on the coat protein sequences.

This study reported for the first time: (i) the development of primers based on the complete CP gene sequences for RT-PCR assays for the universal detection of vitivirus species, (ii) the high genetic variability among Mediterranean isolates of vitiviruses and (iii) the presence of GVD in Jordanian vines, of GVE in grapevines from Hungary, Italy, Jordan, Malta and Palestine, and of GVF in grapevines from Afghanistan, Bulgaria, China, France, Hungary, Italy, Jordan, Lebanon and Malta. PMID: 31400362 [PubMed - as supplied by publisher]
Source: Journal of Virological Methods - Category: Virology Authors: Tags: J Virol Methods Source Type: research