Overexpression of acetyl-CoA carboxylase increases fatty acid production in the green alga Chlamydomonas reinhardtii.

Overexpression of acetyl-CoA carboxylase increases fatty acid production in the green alga Chlamydomonas reinhardtii.
Biotechnol Lett. 2019 Aug 09;:
Authors: Chen D, Yuan X, Liang L, Liu K, Ye H, Liu Z, Liu Y, Huang L, He W, Chen Y, Zhang Y, Xue T
Abstract
Chlamydomonas reinhardtii is a photosynthetic unicellular model algae with multiple biotechnological advantages, and its fatty acids can be used to produce biofuels. Numerous studies suggest that acetyl-coA carboxylase (ACCa) catalyzes the first committed and rate-limiting step of fatty acid biosynthesis, thereby playing a central role in oil accumulation. Here, we cloned and overexpressed ACCa in C. reinhardtii to directly evaluate its effect on fatty acid synthesis. GC-MS analysis found that the unsaturated FAs contents of the CW15-24 and CW15-85 strains were 55.45% and 56.15%, which were significantly enriched compared to the wild type CW15 (48.39%). Under the optimized conditions, the content of lipid by overexpressed the ACCa gene in the mutant CW15-85 (0.46 g/l) was 1.16-fold greater than control through optimization of N and P sources. Altogether, our data clearly demonstrate that ACCa overexpression in C. reinhardtii can directly increase the synthesis of fatty acids.
PMID: 31399913 [PubMed - as supplied by publisher]

Source: Biotechnology Letters - August 8, 2019 Category: Biotechnology Authors: Chen D, Yuan X, Liang L, Liu K, Ye H, Liu Z, Liu Y, Huang L, He W, Chen Y, Zhang Y, Xue T Tags: Biotechnol Lett Source Type: research