Monosynaptic tracing: a step-by-step protocol

Publication date: Available online 10 August 2019Source: Journal of Chemical NeuroanatomyAuthor(s): Thomas K. Lavin, Lei Jin, Ian R. WickershamAbstractMonosynaptic tracing using deletion-mutant rabies virus allows whole-brain mapping of neurons that are directly presynaptic to a targeted population of neurons. The most common and robust way of implementing it is to use Cre mouse lines in combination with Cre-dependent adeno-associated viral vectors for expression of the required genes in the targeted neurons before subsequent injection of the rabies virus. Here we present a step-by-step protocol for performing such experiments using first-generation (ΔG) rabies viral vectors.
Source: Journal of Chemical Neuroanatomy - Category: Neuroscience Source Type: research

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AbstractMonitoring neuronal activityin vivo is critical to understanding the physiological or pathological functions of the brain. Two-photon Ca2+ imagingin vivo using a cranial window and specific neuronal labeling enables real-time,in situ, and long-term imaging of the living brain. Here, we constructed a recombinant rabies virus containing the Ca2+ indicator GCaMP6s along with the fluorescent protein DsRed2 as a baseline reference to ensure GCaMP6s signal reliability. This functional tracer was applied to retrogradely label specific V1 –thalamus circuits and detect spontaneous Ca2+ activity in the dendrites of V1 ...
Source: Neuroscience Bulletin - Category: Neuroscience Source Type: research
Vector-Borne and Zoonotic Diseases, Ahead of Print.
Source: Vector-Borne and Zoonotic Diseases - Category: Infectious Diseases Authors: Source Type: research
In conclusion, our results demonstrate that MPLA serving as an adjuvant enhances the intensity of humoral immune responses by activating the cDC–Tfh–GC B axis. Our findings will contribute to the improvement of the efficiency of traditional rabies vaccines.
Source: Viruses - Category: Virology Authors: Tags: Article Source Type: research
by Bruce A. Kimball, Steven F. Volker, Doreen L. Griffin, Shylo R. Johnson, Amy T. Gilbert Rabies is a zoonotic disease caused by infection with rabies virus, which circulates naturally in several wild carnivore and bat reservoirs in the United States (US). The most important reservoir in the US from an animal and public health perspective is the raccoon (Procyon lotor). To prevent the westward expansion of a significant raccoon rabies epizootic along the eastern seaboard, an operational control program implementing oral rabies vaccination (ORV) has existed in the US since the 1990s. Recently, two vaccine efficacy studies...
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research
ConclusionsWithout strong surveillance systems, rabies-free countries are vulnerable to becoming endemic when incursions happen. To prevent this, it may be necessary to vaccinate at least 38% to 56% of the susceptible dog population depending on the risk of reintroduction and transmission dynamics.
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research
Abstract In the Philippines, ten NTDs are prevalent, but only six namely LF, schistosomiasis, STH, food-borne trematodiases, rabies and leprosy are considered to be of public health importance. The 81 provinces in the country are endemic for at least one of these NTDs. Others may be endemic for two or even more of these diseases. Since 2000, after the Philippines accepted and implemented the WHO guidelines for NTDs prevention, control and elimination, significant progress has been achieved in reducing the magnitude of NTDs endemic in the country". Since 2009, out of 46 filariasis-endemic provinces, the number...
Source: Acta Tropica - Category: Infectious Diseases Authors: Tags: Acta Trop Source Type: research
World-leading vaccine scientists from the University of Bristol are working with one of Vietnam's major vaccine manufacturers, Vabiotech, to share cutting-edge knowledge that could help prevent future global outbreaks of avian flu and rabies.
Source: University of Bristol news - Category: Universities & Medical Training Tags: Health, International, Research; Faculty of Life Sciences, Faculty of Life Sciences, School of Biochemistry, Institutes, Institutes, Bristol BioDesign Institute; Press Release Source Type: news
Publication date: 23 November 2019Source: New Scientist, Volume 244, Issue 3257Author(s): Ruby Prosser Scully
Source: New Scientist - Category: Science Source Type: research
Publication date: Available online 20 November 2019Source: NeuronAuthor(s): Nicholas R. Wall, Peter A. Neumann, Kevin T. Beier, Ava K. Mokhtari, Liqun Luo, Robert C. MalenkaSummaryDrugs of abuse elicit powerful experiences that engage populations of neurons broadly distributed throughout the brain. To determine how synaptic connectivity is organized to enable robust communication between populations of drug-activated neurons, we developed a complementary targeting system for monosynaptic rabies virus (RV) tracing that identifies direct inputs to activated versus nonactivated neuronal populations. Analysis of over 100,000 s...
Source: Neuron - Category: Neuroscience Source Type: research
This study is the first global analysis of lncRNA and mRNA co-expression during RABV infection, provides deeper insight into the mechanism of RABV pathogenesis, and reveals promising candidate for future investigation.
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by array Non-coding RNA profiling by array Homo sapiens Source Type: research
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