Generation of four H1 hESC sublines carrying a hemizygous knock-out/mutant MECP2

Publication date: Available online 9 August 2019Source: Stem Cell ResearchAuthor(s): Ruizhu Zeng, Harwin Sidik, Kim S. Robinson, Franklin L. Zhong, Bruno Reversade, Mahmoud A. PouladiAbstractRett syndrome (RTT) is a childhood neurodevelopmental disorder caused by mutations in MECP2. To study the molecular mechanisms underlying RTT, four sublines of H1 hESCs were generated, carrying a hemizygous knockout or mutant allele of MECP2. Exons 3 and 4 of MECP2 were targeted using the CRISPR/Cas9 nuclease system.
Source: Stem Cell Research - Category: Stem Cells Source Type: research