Calcium Imaging of Microglial Network Activity in Stroke.

This reporter system has enabled in vivo studies of intracellular calcium in large microglial cell populations in cerebral pathologies such as ischemic stroke. In this chapter, we outline specific guidelines for genetic, surgical, imaging, and data analysis aspects of microglial calcium monitoring of the ischemic cortex following middle cerebral artery occlusion. PMID: 31392691 [PubMed - in process]

https://www.ncbi.nlm.nih.gov/pubmed/31392691?dopt=Abstract

Source: Mol Biol Cell - August 9, 2019 Category: Molecular Biology Authors: Tvrdik P, Kearns KN, Sharifi KA, Sluzewski MF, Acton ST, Kalani MYS Tags: Methods Mol Biol Source Type: research