Reaction Mechanisms of Pol IV, RDR2, and DCL3 Drive RNA Channeling in the siRNA-Directed DNA Methylation Pathway

siRNA-directed DNA methylation is important for genome defense against transposons and viruses. Singh et  al. recapitulate siRNA biogenesis in vitro using NUCLEAR RNA POLYMERASE IV, RNA-DEPENDENT RNA POLYMERASE 2, and DICER-LIKE 3, providing mechanistic insights into Pol IV termination, RDR2 activation, siRNA precursor strand specification, and DCL3 dicing.

Source: Molecular Cell - August 7, 2019 Category: Cytology Authors: Jasleen Singh, Vibhor Mishra, Feng Wang, Hsiao-Yun Huang, Craig S. Pikaard Tags: Article Source Type: research