Amentoflavone inhibits tumorsphere formation by regulating the Hedgehog/Gli1 signaling pathway in SUM159 breast cancer stem cells

This study aimed to investigate the effects of amentoflavone, a natural compound identified from Chamaecyparis obtusa, on stemness of basal-like SUM159 breast CSCs, and its underlying mechanism of action. We found that amentoflavone suppressed tumorsphere formation and regulated the expression of the stem cell markers CD44, CD24, and ALDH1 in a dose dependent manner, and inhibited Hedgehog (Hh) signaling by down-regulating Smo, Gli1, OCT4, and NANOG. Knock-down of Gli1 by siRNA significantly affected the expression of CD44, CD24, ALDH1, OCT4, and NANOG, and reduced tumorsphere formation efficiency in SUM159 breast CSCs. Our findings indicate that amentoflavone inhibits SUM159 breast cancer stemness by suppressing Hh/Gli1 signaling, and is a potent agent for targeting breast CSCs.Graphical abstract
Source: Journal of Functional Foods - Category: Nutrition Source Type: research