Fabrication of carbon nanotube-polylactic acid composite electrode by melt compounding for capillary electrophoretic determination of tectoridin and irigenin in Belamcandae Rhizoma

Publication date: Available online 10 July 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Hua Mao, Xinming Ye, Weilong Chen, Wenye Geng, Gang ChenAbstractA sensitive detection electrode based on the composite of carbon nanotubes (CNTs) and polylactic acid (PLA) was fabricated for measuring the bioactive constituents in Belamcandae Rhizoma by capillary electrophoresis (CE). The composite was facilely fabricated by packing a blend of CNTs and melted PLA into a fused silica capillary under heat. The prepared CNT-PLA composite was characterized by scanning electron microscopy, Raman spectroscopy, Fourier transform infrared spectroscopy and cyclic voltammetry. The results indicated that PLA bound electrically conductive CNTs to form a well-dispersed composite network for electrochemical sensing. The electrode was employed to the off-line detection of tectoridin and irigenin in Belamcandae Rhizoma to demonstrate its performance in capillary electrophoresis. At a separation voltage of 12 kV, the two isoflavones were well separated and detected within 8 min in a 40-cm fused silica capillary in a borate buffer at pH 9.8. The detection limits for tectoridin and irigenin were measured to be 0.24 and 0.21 μM, respectively.Graphical AbstractA carbon nanotube-polylactic acid composite electrode was facilely fabricated by melt compounding for enhanced amperometric detection and capillary electrophoretic measurement of tectoridin and irigenin in Belamcandae Rh...
Source: Journal of Pharmaceutical and Biomedical Analysis - Category: Drugs & Pharmacology Source Type: research