Establishment of BV2 microglia polarization model and its effect on Toxoplasma gondii proliferation

Publication date: Available online 5 August 2019Source: Research in Veterinary ScienceAuthor(s): Longtao Wang, Qiang Liu, Yongli Zhang, Lisi Chen, Shouping Hu, Zhuo Zhang, Jingfei Wang, Xuehui Cai, Honglin Jia, Xijun HeAbstractToxoplasma gondii is an intracellular opportunistic, parasitic protozoan. Microglia have been classified into two main types: M1 (classically activated macrophages) and M2 (alternatively activated macrophages). BV2 cells were used in this study, together with lipopolysaccharide (LPS) and interferon (IFN)-γ or interleukin (IL)-4, which were used to induce resting microglia. Expression levels of M1/M2 markers were determined at both mRNA and protein levels, using PCR, western blot, and flow cytometry. Furthermore, cells were infected with T. gondii PLK strain, and the dynamic changes in M1/M2 marker expression levels were determined. An in vitro polarization model was successfully established. Expression of Nos2 and M1-associated markers was significantly upregulated at 12 h post-infection in BV2 cells. Further, the JAK/STAT1 and NF-κB signaling pathways were also activated following T. gondii infection. This demonstrated that T. gondii infection induces M1-type microglial polarization in vitro. The present study demonstrated that T. gondii infection affects microglial activation in vitro and elucidated the effects of activated microglia on T. gondii proliferation. This data may serve as a useful reference for more detailed elucidation of interactions...
Source: Research in Veterinary Science - Category: Veterinary Research Source Type: research