A role for gangliosides and β1-integrin in the motility of olfactory ensheathing glia.

A role for gangliosides and β1-integrin in the motility of olfactory ensheathing glia.
J Anat. 2019 Aug 02;:
Authors: Santos-Silva A, Piña-Rodrigues FM, Mermelstein CDS, Allodi S, Barradas PC, Cavalcante LA
Abstract
Olfactory ensheathing glia (OEG) are found in the olfactory mucosa, nerve and bulb, and provide in vivo ensheathment for the unmyelinated olfactory axons within the central and peripheral nervous system domains. OEG cells are able to migrate long distances within the neuropil of the central nervous system. Because gangliosides such as 9-O-acetyl GD3 have crucial regulatory roles in neuronal migration during development, we analyzed whether OEG in organotypical cultures are revealed by anti-9-O-acetyl GD3 and/or gangliosides are recognized by the A2B5 antibody (G-A2B5), and whether these gangliosides are involved in OEG migration. Our results showed that all OEG migrating out of a section of olfactory bulb onto a laminin substrate bound to the 9-O-acetyl GD3 and A2B5 antibodies, and that 2',3'-cyclic nucleotide phosphodiesterase (CNPase) colocalized with 9-O-acetyl GD3 and with G-A2B5. Additionally, we showed that the immune blockade of 9-O-acetyl GD3 or G-A2B5 reduced the migration of OEG on laminin, and that 9-O-acetyl GD3 and G-A2B5 colocalized with the β1-integrin subunit. We also confirmed the phenotype of in-vitro-grown OEG cells derived from adult rats, showing that they express CNPase, and also α-smooth muscle ...

Source: Journal of Anatomy - August 1, 2019 Category: Anatomy Authors: Santos-Silva A, Piña-Rodrigues FM, Mermelstein CDS, Allodi S, Barradas PC, Cavalcante LA Tags: J Anat Source Type: research