In Vivo Genome-Wide RNA Structure Probing with Structure-seq.

In Vivo Genome-Wide RNA Structure Probing with Structure-seq.
Methods Mol Biol. 2019;1933:305-341
Authors: Ritchey LE, Su Z, Assmann SM, Bevilacqua PC
Abstract
In vivo genome-wide RNA structure probing provides a global view of RNA structure as it occurs in the cell and can assist in elucidating important functional aspects of RNA structure. Structure-seq2 provides high-quality data on transcriptome-wide RNA structure in vivo but contains numerous steps that require technical precision. In this chapter we present the steps needed to produce high-quality structural data with a focus on controls and troubleshooting. Structure-seq2 can be applied to numerous organisms including plants, humans, and bacteria and is amenable to a wide variety of RNA-modifying chemicals including DMS, glyoxal, and SHAPE reagents. Notably, the data generated by the method highlighted here can be readily analyzed using our StructureFold2 computational pipeline.
PMID: 30945195 [PubMed - indexed for MEDLINE]

Source: Mol Biol Cell - August 4, 2019 Category: Molecular Biology Authors: Ritchey LE, Su Z, Assmann SM, Bevilacqua PC Tags: Methods Mol Biol Source Type: research