Analysis of Spatial Assembly of GPCRs Using Photoactivatable Dyes and Localization Microscopy.

Analysis of Spatial Assembly of GPCRs Using Photoactivatable Dyes and Localization Microscopy. Methods Mol Biol. 2019;1947:337-348 Authors: Jonas KC, Hanyaloglu AC Abstract Super-resolution imaging has provided unprecedented insight in the molecular complexities of fundamental cell biological questions. For G protein-coupled receptors (GPCRs), its application to the study of receptor homomers and heteromers have unveiled the diversity of complexes these GPCRs can form at the plasma membrane at a structural and functional level. Here, we describe our methodological approach of photoactivated localization microscopy with photoactivatable dyes (PD-PALM) to visualize and quantify the spatial assembly of GPCR heteromers at the plasma membrane. PMID: 30969426 [PubMed - indexed for MEDLINE]
Source: Mol Biol Cell - Category: Molecular Biology Authors: Tags: Methods Mol Biol Source Type: research