Study on hepatotoxicity of different dosages of Polygoni multiflori radix praeparata in rats by metabolomics based on UPLC-Q-TOF-MS

Publication date: 25 October 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 175Author(s): Xiaohong Gong, Meichen Liu, Lihong Gong, Yunxia Li, Cheng PengAbstractAs a kind of replenishing Chinese medicine, polygoni multiflori radix praeparata (PMP) had high application value in clinic. However, with the increase of clinical applications, more and more hepatotoxicity reports have been reported which had shown has a dose-time-toxicity dependent correlation of PMP hepatotoxicity. Therefore, it was particularly important to investigate the safe and effective dose of PMP in clinical drug administration. At the same time, reliable and sensitive biomarkers were used to characterize phenotypic biochemical disturbances in the body, thereby reflecting the hepatotoxicity mechanism caused by PMP, and providing a basis for clinical drug safety. 10, 20, and 40 g·kg−1 doses PMP were intragastricly administered to rats respectively for consecutive 28 days. serum and liver tissue were collected to measure biochemical markers using blood biochemical analyzers and observe the histopathological examination. Serum metabonomics studies were performed by UPLC-Q-TOF-MS to reveal the dose-dependent biochemical disturbances caused by PMP. Compared with the blank group, the results of biochemical analysis showed that the indicators were significantly changed in the medium dose and high dose groups. however, there was no significant difference in the low dose group. A total of ...
Source: Journal of Pharmaceutical and Biomedical Analysis - Category: Drugs & Pharmacology Source Type: research