Evaluation of white Kwao Krua ( Pueraria candollei Grah. ex Benth.) products sold in Thailand by molecular, chemical, and microscopic analyses

In this study, we authenticated white Kwao Krua products collected from Thai herbal markets by molecular, chemical, and microscopic analyses. The nucleotide sequences in the internal transcribed spacer (ITS) andtrnH –psbA regions of 23 samples of authenticP. candollei were analyzed, and both regions were found to have intraspecific DNA polymorphisms. Based on the single nucleotide polymorphisms in the ITS1 region, species-specific primer sets ofP. candollei were designed to authenticate white Kwao Krua and differentiate it from red and black Kwao Krua. Only the PCR products of KWP02 were not amplified by the primer sets. Isoflavonoid contents and microscopic features were used to support the results of molecular analysis to clarify the botanical origin of white Kwao Krua. Molecular, chemical and microscopic methods confirmed that all the Thai Kwao Krua products examined in this study contained authentic “white Kwao Krua” as claimed on their labels.
Source: Journal of Natural Medicines - Category: Drugs & Pharmacology Source Type: research