Whole-Genome Phage Display Libraries: A Powerful Tool for Antigen Discovery.
Whole-Genome Phage Display Libraries: A Powerful Tool for Antigen Discovery.
Methods Mol Biol. 2019;2024:181-198
Authors: Beghetto E, Gargano N
Abstract
In the last two decades, phage display technology has been used for investigating complex biological processes and isolating molecules of practical value in several applications. Bacteriophage lambda, representing a classical cloning and expression system, has also been exploited for generating display libraries of small peptides and protein domains. More recently, large cDNA and whole-genome lambda display libraries of human pathogens have been generated for the discovery of new antigens for biomedical applications. Here, we describe the construction of a whole-genome library of a common pathogen-Streptococcus pneumoniae-and the use of this library for the molecular dissection of the human B-cell response against bacterial infection and colonization.
PMID: 31364050 [PubMed - in process]
Source: Mol Biol Cell - Category: Molecular Biology Authors: Beghetto E, Gargano N Tags: Methods Mol Biol Source Type: research
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